Intracisternal administration of Mycobacterium tuberculosis potentiates the delayed type hypersensitivity in guinea-pigs.

Physiologia Bohemoslovaca Pub Date : 1990-01-01
I Niedermayerová-Roucová, P Hude, V Strnadová, O Gulda
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Abstract

The delayed type hypersensitivity (DTH) was studied in guinea-pigs using the skin test. The mycobacterium tuberculosis (M. tbc)--was applied by various routes. The control group received ovalbumin in Freund's incomplete adjuvant (FIA) into the footpad. The first experimental group received ovalbumin in Freund's complete adjuvant (FCA) into the footpad. The other experimental groups always received, in addition to ovalbumin plus FIA into the footpad, the M. tbc. 1. intracisternally, 2. intramuscularly, 3. intraperitoneally, 4. orally. On the day of administration of the sensibilizing substance, the body temperature was monitored. The skin test was measured after 14 and 21 days. It was established that, for the study of the DTH, the 21-day interval was more significant than the 14-day interval. A 100 times smaller dose of M. tbc. given intracisternally had the same immunostimulating effect as the injection of ovalbumin with M. tbc into the footpad (p less than 0.01). The size of the skin reaction was not only significantly influenced by the intramuscular and oral administration of M. tbc. On the other hand, the intraperitoneal administration inhibited the DTH (p less than 0.01). The increase of body temperature after the administration of M. tbc. correlated with the influence on the DTH except for the intraperitoneal administration. The route of the M. tbc. administration was crucial for the development of the DTH.

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腹腔注射结核分枝杆菌可增强豚鼠的延迟型超敏反应。
用皮肤试验研究了豚鼠的延迟型超敏反应(DTH)。结核分枝杆菌(M. tbc)通过各种途径应用。对照组给予卵清蛋白不完全佐剂(FIA)注入足垫。第一实验组在足垫内注射卵清蛋白(FCA)。其他实验组除卵清蛋白加FIA外,总接受M. tbc。1. 脑池内的2。肌内,3。腹腔内,4。口服药。在给药当天监测小鼠体温。分别于第14天和第21天进行皮肤试验。结果表明,对于DTH的研究,21天的间隔时间比14天的间隔时间更重要。少了100倍的结核分枝杆菌。腹腔注射与足垫内注射卵清蛋白具有相同的免疫刺激作用(p < 0.01)。皮肤反应的大小不仅受到肌肉注射和口服m.t bc的显著影响。另一方面,腹腔内给药抑制DTH (p < 0.01)。给药后体温升高。除腹腔内给药外,均与DTH的影响相关。地铁的路线。管理对DTH的发展至关重要。
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