[SEM studies on IFN-receptor dynamics of cultured cells from mammals].

Abstract

Visualization of rIFN-alpha 1 and -alpha 2 receptors on MDBK-cells was done by means of a two step method with gold-labelled monoclonal anti IFN-antibodies in the SEM. This binding showed a marked dependency from the temperature, concentration of the rHu-IFN, prefixation of the MDBK-cells, and time of incubation. This results are in agreement with findings of classical virological methods. By means of SEM was shown that the distribution and amount of the IFN-receptors on MDBK-cells depends strongly on the cell cycle and that not each was labelled. The results were discussed in connection with structural-functional alterations of the cytoskeleton.