A Case of a 63-bp Deletion in the mpt64 Gene of Mycobacterium tuberculosis Strains Which Showed False Negativity in the Immunochromatographic Assay

C. Yoon, Y. Hong, Jin Kyung Lee, Yoon Hwan Chang, Seokil Hong
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Abstract

Mycobacterium tuberculosis complex (MTBC) is discriminated from non-tuberculous mycobacteria (NTM) via an immunochromatographic assay (ICA) which is based on the reactions of monoclonal antibodies against MPT64, one of the predominant proteins excreted by MTBC. Recently, the authors of the present study discovered SD TB-negative Mycobacterium tuberculosis strains. In addition, sequence analysis of the mpt64 genes in these strains was performed and showed a deletion of 63 bp from nucleotides 196 to 258. In cases of MPT64-negative mycobacterium, the authors recommend performing TB PCR for correct diagnosis. (Korean J Clin Microbiol 2011;14:36-38)
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结核分枝杆菌mpt64基因63-bp缺失免疫层析检测假阴性1例
结核分枝杆菌复合体(MTBC)通过免疫层析法(ICA)与非结核分枝杆菌(NTM)区分,该方法是基于针对MPT64的单克隆抗体的反应,MPT64是MTBC分泌的主要蛋白质之一。最近,本研究的作者发现了SD结核分枝杆菌阴性结核菌株。此外,对这些菌株的mpt64基因进行了序列分析,发现从核苷酸196到258缺失了63 bp。在mpt64阴性分枝杆菌的病例中,作者建议使用TB PCR进行正确诊断。(中华临床微生物学杂志2011;14:36-38)
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