{"title":"Affinity purification of specific DNA fragments using a lac repressor fusion protein","authors":"Joakim Lundeberg, Johan Wahlberg, Mathias Uhlén","doi":"10.1016/0735-0651(90)90040-M","DOIUrl":null,"url":null,"abstract":"<div><p>A new method for purification of specific DNA sequences using a solid phase technique has been developed based on a fusion between the <em>Escherichia coli lac</em> repressor gene (<em>lac</em><strong>I</strong>) and the staphylococcal protein A gene (<em>spa</em>). The fusion protein, expressed in <em>Escherichia</em><em>coli</em>, is active both <em>in vivo</em> and <em>in vitro</em> with respect to its three functional activities (DNA binding, IPTG induction, and IgG binding). The recombinant protein can be immobilized in a one-step procedure with high yield and parity using the specific interaction between protein A and the Fc-part of immunoglobulin G. The immobilized repressor can thereafter be used for affinity purification of specific DNA fragments containing the <em>lac</em> operator (<em>lacO</em>) sequence.</p></div>","PeriodicalId":77714,"journal":{"name":"Gene analysis techniques","volume":"7 3","pages":"Pages 47-52"},"PeriodicalIF":0.0000,"publicationDate":"1990-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0735-0651(90)90040-M","citationCount":"29","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gene analysis techniques","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/073506519090040M","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 29
Abstract
A new method for purification of specific DNA sequences using a solid phase technique has been developed based on a fusion between the Escherichia coli lac repressor gene (lacI) and the staphylococcal protein A gene (spa). The fusion protein, expressed in Escherichiacoli, is active both in vivo and in vitro with respect to its three functional activities (DNA binding, IPTG induction, and IgG binding). The recombinant protein can be immobilized in a one-step procedure with high yield and parity using the specific interaction between protein A and the Fc-part of immunoglobulin G. The immobilized repressor can thereafter be used for affinity purification of specific DNA fragments containing the lac operator (lacO) sequence.
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