Enhancement of shoot tip multiplication and rooting formation in micropropagation of Yucca elephantipes under different concentrations of BA and IBA.

Abstract

. This study was conducted in the tissue culture laboratory at the Department of Arid Land Agriculture, Faculty of Meteorology, Environment and Arid Land Agriculture, King Abdulaziz University, Jeddah. It aimed to study the effect of BA and IBA concentrations on shoot tips multiplication and rooting formation under Murashige and Skoog medium with Yucca elephantipes micropropagation. The results showed that BA with 2, 3 and 1 mg/l concentrations produced the highest number of developed vegetative branches with values of 8.750, 6815 and 5.625, respectively, but the zero BA did not produce any developed branches. No significant differences were shown for the vegetative branches heights under the effects of the studied BA concentrations (from 1 to 7mg/l). Also, the effects of IBA concentrations on rooting of plantlets of Yucca elephantipes roots under the Murashige and Skoog medium were studied. The results showed that the 1 mg/l IBA produced the highest number of roots (18) and equally significant with the 0.6 mg/l IBA which produced 14 roots. But it differed from the 0.2 and 0.4 mg/l concentrations which produced 10 roots. The highest root length was produced from the concentrations of 1 and 0.4 mg/l IBA with values of 15.666 cm and 14.813 cm, respectively.