Multilevel calcium phosphate coating: A model of bone-like topography for osteoimmunology

PROCEEDINGS OF THE INTERNATIONAL CONFERENCE ON PHYSICAL MESOMECHANICS. MATERIALS WITH MULTILEVEL HIERARCHICAL STRUCTURE AND INTELLIGENT MANUFACTURING TECHNOLOGY Pub Date : 2020-12-14 DOI:10.17223/9785946219242/231

L. Litvinova, V. Malashchenko, E. Shunkin, V. Shupletsova, O. Khaziakhmatova, K. Yurova, E. Melashchenko, I. Khlusov, E. Komarova, V. Chebodaeva, Y. Sharkeev

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Abstract

Osteoimmunolgy describes the interactions between blood immune cells and human multipotent mesenchymal stromal cells (hMMSCs) as a basis of successful wound and fracture healing. The aim was to investigate in vitro interaction of adipose-derived hMMSCs and human blood mononuclear cells (hBMNCs) modulated by calcium phosphate (CaP) coating in 3D culture. Titanium plates (10 × 10 × 1 mm3) with micro-arc bilateral multilevel CaP coatings were used. Cell-IQ phase-contrast microscopy and the real-time cell analyzer (RTCA) showed the CaP coating and hBMNCs synergistic negative effects on hMMSC motility that could be conditioned by enhanced osteogenic differentiation of stromal cells. Indeed, a 10-fold increase in the bone mineralization around the CaP-coated samples was detected in the mixed (hBMNCs + hMMSCs) 3D culture. Thus, cellular and molecular crosstalk between hBMNCs and hMMSCs modulated by multilevel micro-arc CaP coating with bone-like topography is an effective 3D model to study in vitro the novel pathways of osteoimmunology.

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多层磷酸钙涂层:用于骨免疫学的骨样地形模型

骨免疫学描述了血液免疫细胞和人多能间充质间质细胞(hMMSCs)之间的相互作用是伤口和骨折成功愈合的基础。目的是在体外三维培养中研究脂肪源性hMMSCs与磷酸钙(CaP)包被调节的人血单个核细胞(hbmnc)的相互作用。钛板(10 × 10 × 1 mm3)，微弧双侧多层CaP涂层。cell - iq相衬显微镜和实时细胞分析仪(RTCA)显示，CaP涂层和hBMNCs对hMMSC运动的协同负作用可能通过增强基质细胞的成骨分化来调节。事实上，在混合(hbmnc + hMMSCs) 3D培养中，检测到cap涂层样品周围的骨矿化增加了10倍。因此，具有骨状形貌的多层微弧CaP涂层调节hBMNCs和hMMSCs之间的细胞和分子串扰是体外研究骨免疫学新途径的有效3D模型。

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PROCEEDINGS OF THE INTERNATIONAL CONFERENCE ON PHYSICAL MESOMECHANICS. MATERIALS WITH MULTILEVEL HIERARCHICAL STRUCTURE AND INTELLIGENT MANUFACTURING TECHNOLOGY

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