Uji Efektivitas Daya Antibakteri Ekstrak Alga Terhadap Pertumbuhan Bakteri Patogen Streptococcus Mutans

A. Nurfadillah, Juniati Binti Lukman, A. Irma, Miladiarsi, Wahdaniar, Tenri Ayu Adri
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Abstract

Background: Steptoccocus mutans is one of flora normal bacteria in the human body which can cause infections in the mouth cavity. Algae are plant-like organisms that may be found in a marine ecosystem. Recents studies have reported that algae contains numerous pharmacological activities compounds, such us: antibiotic, cytotoxic, antifungal, anti virus and antitumor. Purpose: this research aimed to know the  antibacterial activity of algae extract against the growth of pathogen bacteria Streptococcus mutans. Methods: Algae were extracted by using aquades.The inhibition test was performed by agar diffusion method on Glucose Nutrient Agar (GNA) using three types of algae extracts: Eucheuma cottoni, Turbinaria decurrens and Ulva reticulata on the incubation period 1x24 and 2x24 hours at 37◦C. Data collection technique was based on the results of measuring the diameter of the inhibition zone (clear zone) for the growth of Streptococcus mutans bacteria using a caliper. The data obtained were analyzed using quantitative descriptive analysis methods. This data analysis was processed to see if there was a difference in antibacterial power from extract algae samples. Results: U. Reticulate algae extract formed a clear zone 11.2 mm in diameter at 1x24-hour incubation, and 10.3 mm at 2x24-hour incubation. T. deccurens formed clear zones 12.7 mm in diameter at 1x24-hour incubation, and 11.4 mm at 2x24-hour incubation. E. cottonii formed clear zones 10.1 mm in diameter at 1x24-hour incubation, and 9.3 mm at 2x24-hour incubation. Conclusion: Extracts of T. decurrens have the most potential in inhibiting the growth of pathogenic microbes of S. mutants.
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测试藻类提取物对链球菌病原体生长的抗菌作用
背景:变形链球菌是人体正常菌群之一,可引起口腔感染。藻类是一种类似植物的生物,可以在海洋生态系统中找到。近年来的研究报道,藻类含有多种具有药理活性的化合物,如:抗生素、细胞毒、抗真菌、抗病毒和抗肿瘤。目的:研究海藻提取物对致病菌变形链球菌生长的抑菌作用。方法:采用水凝胶法提取藻类。在葡萄糖营养琼脂(Glucose Nutrient agar, GNA)上采用琼脂扩散法,用真毛草(Eucheuma cottoni)、杜鹃(Turbinaria decurrens)和网状藻(Ulva reticulata)三种藻类提取物,37℃孵育1x24和2x24小时进行抑制试验。数据采集技术是基于用卡尺测量变形链球菌生长抑制区(透明区)直径的结果。所得资料采用定量描述性分析方法进行分析。对这些数据进行了分析,以确定提取藻类样品的抗菌能力是否存在差异。结果:U.网状藻提取物在孵育1 × 24小时时形成直径11.2 mm的清晰带,在孵育2 × 24小时时形成直径10.3 mm的清晰带。在1 × 24小时的孵育过程中,赤斑绦虫形成了直径12.7 mm的清晰区,在2 × 24小时的孵育过程中形成了直径11.4 mm的清晰区。在孵育1 × 24小时时,棉铃虫形成直径10.1 mm的清晰带,在孵育2 × 24小时时形成直径9.3 mm的清晰带。结论:延胡索提取物对突变葡萄球菌病原菌生长的抑制作用最大。
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