Radiobiological dose-response relationships by monitoring the ATP-concentration in L 1210 cells using 31P-NMR-spectroscopy.

Abstract

In conventional radiobiology the survival fraction of cell cultures in the exponential phase is observed by the colony-forming ability, and the linear-quadratic model represents an adequate frame of the numerical adaptation. The measurement of the ATP-concentration of L 1210 cells by 31P-NMR-spectroscopy in dependence of the applied radiation dose can also be used to evaluate dose-response relationships. In particular, the beta-peak of the ATP is suitable to seize the chronological behavior of the cellular ATP-concentration. An access to RBE of a radiation quality is obtained by monitoring the ATP-concentration, and the combination treatment (irradiation and incubation of cisPlatinum) has also been studied with regard to the question of synergistic interactions.