Correlation between Urinary Cyclophilin A and Urinary Albumin Levels on Diabetic Kidney Disease

Abstract

Introduction: Diabetic kidney disease (DKD) is a complication of diabetes mellitus characterized by albuminuria persisting within 3 to 6 months, the earliest clinical evidence is microalbuminuria (30-299 mg/24hours or 20-199 ug/i). Cyclophilin A (Cyp A) is an 18 k-Da 165-amino acid long cytosolic protein also known as peptidylprolyl isomerase A. In DN, hyperglycemia will cause Cyp A secretion by human kidney-2 (HK-2) cells from PTEC and mesangial-13 cells (MES-13) and causes kidney damage. Material and Methods: This study was an analytic observational, cross sectional study conducted at the clinic and inpatient internal medicine installation at dr. M. Djamil General Hospital Padang  for 6 months. Samples were selected by consecutive sampling, as many as 60 people with post prandial blood glucose > 180 mg/dl and urinary albumin > 30 mg/24 hours and met the inclusion and exclusion criteria. The samples were examined for urinary Cyp A and albumin levels. Results: The mean level of urinary Cyp A in patients with DKD is 4.96 (2.03) ng/ml. Median urinary albumin levels in DKD patients is 287.89 (30.79-394.57) mg/24 hours. Correlation analysis between urinary Cyp A and albumin levels showed a significant (p < 0.05) with a positive and strong correlation (r = 0.776) in DKD patients. Conclusion: There was an increase of urinary Cyp A and urinary albumin levels, with a positive and strong correlation between them in DKD patients. Keywords: Urinary Cyclophilin A, urinary albumin, diabetic kidney disease