V. Krnjaja, T. Petrović, S. Stankovic, M. Lukić, Z. Škrbić, Violeta Mandić, Z. Bijelić
{"title":"Mycobiota and aflatoxin B1 in poultry feeds","authors":"V. Krnjaja, T. Petrović, S. Stankovic, M. Lukić, Z. Škrbić, Violeta Mandić, Z. Bijelić","doi":"10.2298/bah1901061k","DOIUrl":null,"url":null,"abstract":"In this study, a total of 30 poultry (chicken and laying hens) feed samples\n collected from different poultry farms in Serbia in 2016 were tested for\n fungal and aflatoxin contamination. Using the plate count and standard\n mycological methods, total fungal counts and potentially toxigenic fungal\n genera were determined. Natural occurrence of aflatoxin B1 (AFB1) was\n detected by ELISA (enzyme-linked immune sorbent assay) method. The total\n fungal count was in the range from 1 x 102 (2 log CFU g-1) to 1.83 x 105 CFU\n g-1 (5.26 log CFU g-1). The majority of the chicken feeds (78.57%) had the\n total fungal count in the ranged from 1 x 102 to 4.8 x 104 CFU g-1, whereas\n in 68.75% of the laying hens feeds it was ranged from 5.3 x 104 to 1.83 x\n 105 CFU g-1. In 21.43% of the chicken feeds fungal contamination reached the\n level above the regulation limits. Three potentially toxigenic fungal\n genera, Aspergillus, Fusarium, and Penicillium, have been identified. In the\n tested poultry feed samples, more samples contaminated with Aspergillus were\n determined compared to samples contaminated by Fusarium and Penicillium\n species. The AFB1 was detected in concentrations from 1.34 to 18.29?g kg-1,\n with an average of 4.47 and 4.56?g kg-1 in the chicken and laying hens feed\n samples, respectively. In 14.29% of the chicken feeds, the level of AFB1 was\n above the regulation limits. The obtained results confirmed the importance\n of continuous mycological and mycotoxicological control of poultry feed, as\n well as need to improve risk assessments of such contaminants along the food\n chain.","PeriodicalId":249404,"journal":{"name":"Biotehnologija u stocarstvu","volume":"9 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biotehnologija u stocarstvu","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2298/bah1901061k","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
In this study, a total of 30 poultry (chicken and laying hens) feed samples
collected from different poultry farms in Serbia in 2016 were tested for
fungal and aflatoxin contamination. Using the plate count and standard
mycological methods, total fungal counts and potentially toxigenic fungal
genera were determined. Natural occurrence of aflatoxin B1 (AFB1) was
detected by ELISA (enzyme-linked immune sorbent assay) method. The total
fungal count was in the range from 1 x 102 (2 log CFU g-1) to 1.83 x 105 CFU
g-1 (5.26 log CFU g-1). The majority of the chicken feeds (78.57%) had the
total fungal count in the ranged from 1 x 102 to 4.8 x 104 CFU g-1, whereas
in 68.75% of the laying hens feeds it was ranged from 5.3 x 104 to 1.83 x
105 CFU g-1. In 21.43% of the chicken feeds fungal contamination reached the
level above the regulation limits. Three potentially toxigenic fungal
genera, Aspergillus, Fusarium, and Penicillium, have been identified. In the
tested poultry feed samples, more samples contaminated with Aspergillus were
determined compared to samples contaminated by Fusarium and Penicillium
species. The AFB1 was detected in concentrations from 1.34 to 18.29?g kg-1,
with an average of 4.47 and 4.56?g kg-1 in the chicken and laying hens feed
samples, respectively. In 14.29% of the chicken feeds, the level of AFB1 was
above the regulation limits. The obtained results confirmed the importance
of continuous mycological and mycotoxicological control of poultry feed, as
well as need to improve risk assessments of such contaminants along the food
chain.