Antioxidant Activity Test on Ethanol Extract Of Soursop Leaves (Annona muricata L.) Using DPPH Method (1,1-diphenyl-2-picrylhidrazyl)

A. Gani, Ratih Delviyanti, R. Rusman
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引用次数: 1

Abstract

Soursop plant parts that are often used by the community, namely the fruit and leaves. Ripe soursop fruit is usually developed to produce juice and lunkhead products. Ripe soursop fruit contains vitamin C which is known to have a wrong function, namely to increase immunity, so that it can protect the body from pathogens. Soursop leaves are often used by the community in the form of a decoction of the leaves for traditional medicine. The research about antioxidant test on ethanol extract of soursop leaves (Annona muricata Linn) had been conducted using DPPH method (1,1-diphenyl-2-picrylhdirazyl). This research aims to indicate the antioxidant activity of soursop leaves. Samples were collected from Lambaro Skep, a region of Banda Aceh. Then, the sample was dried by air before macerated using ethanol 96%. After that, the filtrate is evaporated using rotary evaporator and concentrated again using a hairdryer. The concentrated extract is then tested for antioxidant activity using DPPH method at maximum wavelength of 517 nm. The result shows young soursop leaves extract has 14.462 ppm of IC50 value, while old soursop leaves extract has IC50 value of 9.626 ppm.
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番荔枝叶乙醇提取物的抗氧化活性试验DPPH法(1,1-二苯基-2-苦味基肼基)
番荔枝的植物部分是社区经常使用的,即果实和叶子。成熟的酸菜果实通常被开发成果汁和钝头产品。成熟的番荔枝果实中含有维生素C,而众所周知,维生素C有一个错误的功能,即提高免疫力,从而保护身体免受病原体的侵害。番荔枝的叶子经常被社区用来制作传统药物的汤剂。采用DPPH法(1,1-二苯基-2-苦味基-二嗪基)对番荔枝叶乙醇提取物进行了抗氧化试验研究。本研究的目的是研究刺蒺藜叶的抗氧化活性。样本采集自班达亚齐省Lambaro Skep地区。然后将样品风干,再用96%的乙醇浸泡。之后,滤液用旋转蒸发器蒸发,再用吹风机浓缩。然后用DPPH法在最大波长517 nm处测试浓缩提取物的抗氧化活性。结果表明,幼刺果叶提取物的IC50值为14.462 ppm,老刺果叶提取物的IC50值为9.626 ppm。
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