Detection of Virulence Genes of Proteus Mirabilis Isolated from Clinical Samples of some Hospitals in Nasiriyah City

Pakistan Journal of Medical & Health Sciences Pub Date : 2022-10-30 DOI:10.53350/pjmhs221610433

Amany Shakeir Jaber

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Abstract

The study aimed to detect some virulence genes of Proteus mirabilis isolates, isolation and diagnosis by biochemical tests and Vitek-2 system . The research was conducted in Nasiriyah City/Dhi-Qar, Iraq, in the period between April 2021 and January 2022, and 70 urine and 30 wound samples were obtained from patients admitted to some Hospitals . Isolates diagnosis was made based on morphological and cultural characteristics of blood agar, MacConkey agar, as well as conventional biochemical tests and Vitek-2 identification system. Depending on these criteria, out of 100 samples bacterial growth appeared in only 80 (80.0%) samples, while no bacterial growth appeared in the remaining 20 (20.0%). only 20 (20%) isolates were obtained from P. mirabilis. chosen for genetic testing in order to detect the virulence factors (15) isolates from Proteus mirabilis, such as colonization factor antigen (CFA), extracellular protease, swarming activity, and urease, were identified. The results showed that all isolates (100%) possessed UreC gene, ZapA gene, flaA gene and mrp gene. Keywords: Proteus mirabilis, UTIs, UreC, ZapA, mrp and flaA

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纳西里耶市部分医院临床标本中奇异变形杆菌毒力基因的检测

本研究旨在检测奇异变形杆菌分离株的部分毒力基因，通过生化试验和Vitek-2系统进行分离和诊断。该研究于2021年4月至2022年1月期间在伊拉克纳西里耶市/迪卡尔进行，从一些医院收治的患者中获得了70份尿液和30份伤口样本。根据血琼脂、麦康基琼脂的形态和培养特征，以及常规生化试验和Vitek-2鉴定系统对分离株进行诊断。根据这些标准，100个样品中只有80个(80.0%)样品出现细菌生长，其余20个(20.0%)样品未出现细菌生长。仅有20株(20%)分离株。对15株奇异变形杆菌分离株的定殖因子抗原(CFA)、胞外蛋白酶、蜂群活性和脲酶等毒力因子进行了基因检测。结果表明，所有分离株(100%)均含有UreC基因、ZapA基因、flaA基因和mrp基因。关键词:奇异变形杆菌，uti, UreC, ZapA, mrp, flaA

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Pakistan Journal of Medical & Health Sciences

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