THE EFFECT OF BETA - ARRESTIN 1 TRANSFECTION ON PROLIFERATION AND TEMOZOLOMIDE TREATMENT RESPONSE IN HGG CELLS

Abstract

While several studies have indicated a major role of the ?-arrestin family in the development of several types of cancer, little to no information is available on its influence in malignant gliomas. In our study we transfected an established high-grade glioma cell line, 11 HGG with a ?-1 arrestin plasmid in order to observe how ?-1 overexpression influences proliferation and response to standard of care treatment. After 24h, the transfected cells presented a drop in proliferation when compared to untransfected cells. In terms of treatment response, transfected cells presented a markedly elevated cytotoxic effect when compared to untransfected cells. However, after 48h and 72h transfected cells presented only a minor increase in proliferation while treatments responses to TMZ were modestly influenced by ?-1 arrestin overexpression.