Potential of Human Urine as a Nutrient Medium for the Biomass Production of Microalga Scenedesmus sp

Abebe Muluye, Berhanu Sali, Blien Bahta, B. Melese, Bethel Girma, M. Kebede, Muluken Kebede, A. Suresh
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Abstract

Microalgae have value-added chemicals, but their biomass production is costly due to the expensive nutrient chemicals. Meantime human urine (HU) is considered wastewater and has basic nutrients for autotrophs. This study tested the potentials of being cheaply available in HU as a nutrient source for the biomass production of microalga Scenedesmus sp. HU was collected, sterilized, and made different dilutions. Microalga of Scenedesmus sp., was isolated from the urine contaminated site, purified, inoculated into the HU medium, and incubated under Sunlight for 7 days at 25 °C. The maximum growth was observed in 15 % HU as 0.795 OD670nm with 0.57 g/L biomass production with a significant difference (p<0.01). The productivity of 81 mg/L/day was reached. The concentrated urine of more than 20 % was not supporting the growth of microalga. This study concluded that human urine can be used as a nutrient medium for microalgae growth at certain dilution.
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人尿作为景观微藻生物质生产营养培养基的潜力
微藻具有高附加值的化学物质,但由于其营养化学物质昂贵,其生物质生产成本很高。同时,人尿(HU)被认为是废水,具有自养生物的基本营养。本研究通过收集、灭菌和不同的稀释,测试了HU作为微藻(Scenedesmus sp.)生物质生产的廉价营养源的潜力。从尿污染部位分离出Scenedesmus sp.微藻,纯化后接种于HU培养基中,在25°C的日光下培养7 d。在15% HU中,最大生长量为0.795 OD670nm,生物量产量为0.57 g/L,差异极显著(p<0.01)。产率达到81 mg/L/d。浓度超过20%的尿液不支持微藻的生长。本研究认为,在一定的稀释度下,人尿可以作为微藻生长的营养培养基。
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