Additive and non-additive genetic variance in juvenile Sitka spruce (Picea sitchensis Bong. Carr).

Abstract

Many quantitative genetic models assume that all genetic variation is additive because of a lack of data with sufficient structure and quality to determine the relative contribution of additive and non-additive variation. Here the fractions of additive (f_a) and non-additive (f_d) genetic variation were estimated in Sitka spruce for height, bud burst and pilodyn penetration depth. Approximately 1500 offspring were produced in each of three sib families and clonally replicated across three geographically diverse sites. Genotypes from 1525 offspring from all three families were obtained by RADseq, followed by imputation using 1630 loci segregating in all families and mapped using the newly developed linkage map of Sitka spruce. The analyses employed a new approach for estimating f_a and f_d, which combined all available genotypic and phenotypic data with spatial modelling for each trait and site. The consensus estimate for f_a increased with age for height from 0.58 at 2 years to 0.75 at 11 years, with only small overlap in 95% support intervals (I₉₅). The estimated f_a for bud burst was 0.83 (I₉₅=[0.78, 0.90]) and 0.84 (I₉₅=[0.77, 0.92]) for pilodyn depth. Overall, there was no evidence of family heterogeneity for height or bud burst, or site heterogeneity for pilodyn depth, and no evidence of inbreeding depression associated with genomic homozygosity, expected if dominance variance was the major component of non-additive variance. The results offer no support for the development of sublines for crossing within the species. The models give new opportunities to assess more accurately the scale of non-additive variation.

Supplementary information: The online version contains supplementary material available at 10.1007/s11295-023-01627-5.