Micro-polymerase chain reaction for point-of-care detection and beyond: a review microfluidics and nanofluidics

IF 2.3 4区 工程技术 Q2 INSTRUMENTS & INSTRUMENTATION Microfluidics and Nanofluidics Pub Date : 2023-09-11 DOI:10.1007/s10404-023-02677-w
Gamze Tilbe İnce, Mehmet Yüksekkaya, Orhan Erdem Haberal
{"title":"Micro-polymerase chain reaction for point-of-care detection and beyond: a review microfluidics and nanofluidics","authors":"Gamze Tilbe İnce,&nbsp;Mehmet Yüksekkaya,&nbsp;Orhan Erdem Haberal","doi":"10.1007/s10404-023-02677-w","DOIUrl":null,"url":null,"abstract":"<div><p>Miniaturization of biological and chemical analytical devices with microelectromechanical systems (MEMS) technology is important for medical diagnosis, microbial recognition, and other biological analysis. Current or emerging infectious diseases increase the need for point-of-care testing (POCT) to increase timely diagnosis and treatment. Among the various nucleic acid amplification methods, polymerase chain reaction (PCR) has been the most used method due to its simplicity. MEMS and microfluidic technologies enable PCR processes to be miniaturized in a chip. A miniaturized microfluidic chip is a small device that can limit and flow a specific volume of fluid into micro-sized channels. Microfluidic chip has potential benefits such as speed, cost, portability, efficiency, and automation. In addition to these benefits, multifunctional POCT PCR devices based on microfluidics technology can help with clinical diagnosis in underdeveloped nations that need a centralized health care system. In this review, conventional PCR method and the recent advances in microfluidic PCR amplification technologies, including its usage for POCT, are discussed. Current studies in commercialization of microfluidic PCR devices are presented. POCT has become very important during the Coronavirus disease (COVID-19) epidemic; therefore, the applications that evolved with combined use of POCT, PCR, and microfluidics during the COVID-19 pandemic were also covered.</p></div>","PeriodicalId":706,"journal":{"name":"Microfluidics and Nanofluidics","volume":null,"pages":null},"PeriodicalIF":2.3000,"publicationDate":"2023-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microfluidics and Nanofluidics","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s10404-023-02677-w","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INSTRUMENTS & INSTRUMENTATION","Score":null,"Total":0}
引用次数: 0

Abstract

Miniaturization of biological and chemical analytical devices with microelectromechanical systems (MEMS) technology is important for medical diagnosis, microbial recognition, and other biological analysis. Current or emerging infectious diseases increase the need for point-of-care testing (POCT) to increase timely diagnosis and treatment. Among the various nucleic acid amplification methods, polymerase chain reaction (PCR) has been the most used method due to its simplicity. MEMS and microfluidic technologies enable PCR processes to be miniaturized in a chip. A miniaturized microfluidic chip is a small device that can limit and flow a specific volume of fluid into micro-sized channels. Microfluidic chip has potential benefits such as speed, cost, portability, efficiency, and automation. In addition to these benefits, multifunctional POCT PCR devices based on microfluidics technology can help with clinical diagnosis in underdeveloped nations that need a centralized health care system. In this review, conventional PCR method and the recent advances in microfluidic PCR amplification technologies, including its usage for POCT, are discussed. Current studies in commercialization of microfluidic PCR devices are presented. POCT has become very important during the Coronavirus disease (COVID-19) epidemic; therefore, the applications that evolved with combined use of POCT, PCR, and microfluidics during the COVID-19 pandemic were also covered.

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
微聚合酶链反应用于即时检测及其他:微流体和纳米流体的综述
采用微机电系统(MEMS)技术的生物和化学分析设备的小型化对于医学诊断、微生物识别和其他生物分析非常重要。当前或新出现的传染病增加了对护理点检测(POCT)的需求,以增加及时诊断和治疗。在各种核酸扩增方法中,聚合酶链反应(PCR)因其简便而成为应用最多的方法。MEMS和微流体技术使PCR过程在芯片中小型化。微型微流控芯片是一种可以限制特定体积的流体流入微尺寸通道的小型装置。微流控芯片具有速度快、成本低、便携、高效、自动化等优点。除了这些好处之外,基于微流体技术的多功能POCT PCR装置可以帮助需要集中医疗保健系统的不发达国家进行临床诊断。本文综述了传统PCR方法和微流控PCR扩增技术的最新进展,包括其在POCT中的应用。介绍了目前微流控PCR装置商业化的研究情况。POCT在冠状病毒病(COVID-19)流行期间变得非常重要;因此,在COVID-19大流行期间,POCT、PCR和微流控技术的结合应用也被涵盖。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Microfluidics and Nanofluidics
Microfluidics and Nanofluidics 工程技术-纳米科技
CiteScore
4.80
自引率
3.60%
发文量
97
审稿时长
2 months
期刊介绍: Microfluidics and Nanofluidics is an international peer-reviewed journal that aims to publish papers in all aspects of microfluidics, nanofluidics and lab-on-a-chip science and technology. The objectives of the journal are to (1) provide an overview of the current state of the research and development in microfluidics, nanofluidics and lab-on-a-chip devices, (2) improve the fundamental understanding of microfluidic and nanofluidic phenomena, and (3) discuss applications of microfluidics, nanofluidics and lab-on-a-chip devices. Topics covered in this journal include: 1.000 Fundamental principles of micro- and nanoscale phenomena like, flow, mass transport and reactions 3.000 Theoretical models and numerical simulation with experimental and/or analytical proof 4.000 Novel measurement & characterization technologies 5.000 Devices (actuators and sensors) 6.000 New unit-operations for dedicated microfluidic platforms 7.000 Lab-on-a-Chip applications 8.000 Microfabrication technologies and materials Please note, Microfluidics and Nanofluidics does not publish manuscripts studying pure microscale heat transfer since there are many journals that cover this field of research (Journal of Heat Transfer, Journal of Heat and Mass Transfer, Journal of Heat and Fluid Flow, etc.).
期刊最新文献
Visualizing conformance control mechanisms in high-temperature reservoirs: a microfluidic analysis of Pickering emulsified gel systems Exploring fluid flow in microchannels with branching and variable constrictions Variance-reduction kinetic simulation for characterization of surface and corner effects in low-speed rarefied gas flows through long micro-ducts Dynamic response of a weakly ionized fluid in a vibrating Riga channel exposed to intense electromagnetic rotation Physiological hypoxia promotes cancer cell migration and attenuates angiogenesis in co-culture using a microfluidic device
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1