Biocontrol potential of native entomopathogenic nematodes against Gonioctena fornicata (Brüggemann, 1873) (Coleoptera, Chrysomelidae) in alfalfa

Abstract

Abstract Background Gonioctena fornicata (Brüggemann, 1873) (Coleoptera: Chrysomelidae) inflicts substantial crop damage by consuming alfalfa leaves. The main aim of the study was to develop an efficient, sustainable control method against the pest, considering entomopathogenic nematodes (EPNs) as a potential control agent against soil-dwelling insect pests. This study investigated the impact of two native entomopathogenic nematodes (EPNs), Heterorhabditis bacteriophora (isolate Z-1) and Steinernema feltiae (isolate A-16), on mature larvae and adults of G. fornicata . Concentrations of 250, 500, and 1000 IJs/ml were used for adult and larval stages, and mortality rate was determined at the end of 144 h for larvae and 240 h for adults. Results The highest effect was 94.7% at 120 h for isolate Z-1 and 91.0% at 168 h for isolate A-16 at a concentration of 1000 IJs/ml for adult stages. The highest effect on larval stage was 97.6% at 96 h for isolate Z-1 and 85.8% at 120 h for isolate A-16 at the concentration of 1000 IJs/ml. On the adult stage, isolate Z-1 showed 50.0–67.1% effect at concentrations of 250 and 500 IJs/ml, respectively, while isolate A-16 showed an effect ranging from 46.7 to 63.6%. In the larval stage, isolate Z-1 caused 39.7–77.6% mortality at 250 and 500 IJs/ml concentrations, respectively, while the rate was 32.9–63.9% for isolate A-16. In the trial, isolate Z-1 showed a high efficacy in both stages of G. fornicata . Conclusions This study represents the first laboratory investigation encompassing both adult and mature larval stages of G. fornicata . The obtained data offer valuable insights for alternative control strategies targeting G. fornicata .