Anti-tumor effect of Fasudil, a ROCK inhibitor, on gastric cancer in mice

IF 2 Q2 MEDICINE, GENERAL & INTERNAL International Journal of Health Sciences-IJHS Pub Date : 2023-11-14 DOI:10.53730/ijhs.v7n3.14624
Chengcheng Yang, Yan Miao, Qiang Yu, Xiangjie Wang
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Abstract

Gastric cancer remains one of the most common malignant tumors and a leading cause of death. However, there are few reports about the anti-tumor effect of Fasudil (Fas) on the stomach. Establishment of nude mice model of gastric cancer to evaluate the anti-tumor effect of Fas. The mice in each group were weighed every week. Serum and tumor tissue cytokines are detected by commercial kits. Serum and tumor tissue superoxide dismutase and malondialdehyde were detected by commercial kits. Histopathological changes of the tumor were detected by HE staining. Expression of ROS/ TXNIP/NLRP3/Caspase-1 pathway in tumor tissues was detected by Western blot. Our results showed that Fas increased and decreased the body weight of tumor mice, increased serum and tumor tissue cytokines contents of tumor mice, increased serum and tumor tissue oxidative stress of tumor mice, and increased Bax, Caspase-3, Caspase-9 protein expression, reduced Bcl-2 protein expression, also regulated ROS/TXNIP/NLRP3/Caspase-1 pathway expression. Our experiments show that Fas has a significant anti-gastric cancer effect, and its mechanism is related to the regulation of oxidative stress, inflammation and apoptosis in vivo.
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ROCK抑制剂法舒地尔对小鼠胃癌的抗肿瘤作用
胃癌仍然是最常见的恶性肿瘤之一,也是导致死亡的主要原因。然而,关于法舒地尔(Fas)对胃的抗肿瘤作用的报道很少。建立胃癌裸鼠模型,评价Fas的抗肿瘤作用。每组小鼠每周称重。血清和肿瘤组织细胞因子检测试剂盒。血清及肿瘤组织超氧化物歧化酶、丙二醛检测试剂盒。HE染色检测肿瘤组织病理变化。Western blot检测肿瘤组织中ROS/ TXNIP/NLRP3/Caspase-1通路的表达。我们的研究结果表明,Fas增加和降低肿瘤小鼠的体重,增加肿瘤小鼠血清和肿瘤组织细胞因子含量,增加肿瘤小鼠血清和肿瘤组织氧化应激,增加Bax、Caspase-3、Caspase-9蛋白表达,降低Bcl-2蛋白表达,调节ROS/TXNIP/NLRP3/Caspase-1通路表达。我们的实验表明,Fas具有明显的抗胃癌作用,其机制可能与体内氧化应激、炎症和细胞凋亡的调节有关。
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来源期刊
International Journal of Health Sciences-IJHS
International Journal of Health Sciences-IJHS MEDICINE, GENERAL & INTERNAL-
自引率
15.00%
发文量
49
审稿时长
8 weeks
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