{"title":"Transcriptome Analysis of Gill Tissues from Neptunea cumingii in Different Seasons","authors":"Yifan Zhang, Dandan Zhang, Ying Tian, Junxia Mao, Yang Liu, Zhenlin Hao","doi":"10.3390/fishes8110549","DOIUrl":null,"url":null,"abstract":"Neptunea cumingii is an economically important marine shellfish found in the Yellow and Bohai Seas areas of China. In this study, samples of Neptunea cumingii were collected in Zhangzidao and Yantai during spring, summer, autumn, and winter to clarify the gene expression patterns and regulatory mechanisms in their gills in different seasons. Transcriptome analysis was conducted using Neptunea cumingii gill tissues, and genes with significantly different expression levels were extracted for functional verification. The most genes with differences in expression (DEGs) were found in comparisons of the winter and summer samples. Gene enrichment analysis based on Gene Ontology and Kyoto Encyclopedia of Genes and Genomes terms showed that these DEGs were mainly involved in immune and metabolic pathways, and they had significant effects on oxidative stress, body metabolism, and protein synthesis in Neptunea cumingii. Further screening of DEGs identified thirty-four genes related to temperature regulation, comprising thirteen genes with roles in innate immunity in shellfish, twelve genes related to oxidative stress, and nine genes related to protein synthesis and energy metabolism. Eleven DEGs were randomly selected for qPCR verification, and the results were consistent with the transcriptome analysis results. In summary, the transcriptome results differed significantly between seasons in the gill tissues of Neptunea cumingii. The expression levels of immune regulatory genes could be promoted in Neptunea cumingii during the high temperature season, whereas the expression of these genes may be inhibited in the low temperature season. The results obtained in this study provide insights into the molecular defense mechanisms that might allow Neptunea cumingii to adapt to climate change.","PeriodicalId":12405,"journal":{"name":"Fishes","volume":null,"pages":null},"PeriodicalIF":2.1000,"publicationDate":"2023-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fishes","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3390/fishes8110549","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FISHERIES","Score":null,"Total":0}
引用次数: 0
Abstract
Neptunea cumingii is an economically important marine shellfish found in the Yellow and Bohai Seas areas of China. In this study, samples of Neptunea cumingii were collected in Zhangzidao and Yantai during spring, summer, autumn, and winter to clarify the gene expression patterns and regulatory mechanisms in their gills in different seasons. Transcriptome analysis was conducted using Neptunea cumingii gill tissues, and genes with significantly different expression levels were extracted for functional verification. The most genes with differences in expression (DEGs) were found in comparisons of the winter and summer samples. Gene enrichment analysis based on Gene Ontology and Kyoto Encyclopedia of Genes and Genomes terms showed that these DEGs were mainly involved in immune and metabolic pathways, and they had significant effects on oxidative stress, body metabolism, and protein synthesis in Neptunea cumingii. Further screening of DEGs identified thirty-four genes related to temperature regulation, comprising thirteen genes with roles in innate immunity in shellfish, twelve genes related to oxidative stress, and nine genes related to protein synthesis and energy metabolism. Eleven DEGs were randomly selected for qPCR verification, and the results were consistent with the transcriptome analysis results. In summary, the transcriptome results differed significantly between seasons in the gill tissues of Neptunea cumingii. The expression levels of immune regulatory genes could be promoted in Neptunea cumingii during the high temperature season, whereas the expression of these genes may be inhibited in the low temperature season. The results obtained in this study provide insights into the molecular defense mechanisms that might allow Neptunea cumingii to adapt to climate change.
海螺是一种重要的经济海洋贝类,产于中国黄渤海海域。本研究分别于春、夏、秋、冬采集漳子岛和烟台的三角帆鱼样本,研究不同季节三角帆鱼鳃基因表达模式及调控机制。利用三角帆鱼鳃组织进行转录组分析,提取表达水平差异显著的基因进行功能验证。在冬季和夏季样品的比较中发现了最多的表达差异基因(DEGs)。基于Gene Ontology和Kyoto Encyclopedia of Genes and Genomes术语的基因富集分析表明,这些deg主要参与免疫和代谢途径,对cumingii的氧化应激、机体代谢和蛋白质合成有显著影响。进一步筛选的deg鉴定出34个与温度调节相关的基因,其中13个基因与贝类先天免疫有关,12个基因与氧化应激有关,9个基因与蛋白质合成和能量代谢有关。随机选取11个deg进行qPCR验证,结果与转录组分析结果一致。综上所述,在不同季节,三角帆鱼鳃组织的转录组结果有显著差异。免疫调节基因在高温季节的表达水平可以提高,而在低温季节的表达水平可能会受到抑制。本研究获得的结果为海王星cumingii适应气候变化的分子防御机制提供了见解。