Antigout Activity of The Spatholobus littoralis Hassk. Extract Fractions Against Xanthine Oxidase: Its Metabolite Profile and Inhibition Kinetics

Q3 Agricultural and Biological Sciences HAYATI Journal of Biosciences Pub Date : 2023-09-19 DOI:10.4308/hjb.31.1.1-20

Rut Novalia Rahmawati Sianipar, Komar Sutriah, Dyah Iswantini, None Trivadila, Suminar Setiati Achmadi

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Abstract

The Spatholobus littoralis Hassk. (S. littoralis Hassk.) is a native plant in Indonesia and has been widely used in traditional Dayaknese medicine for non-communicable degenerative diseases. One of these illnesses, known as gout, is caused by excessive uric acid in the blood, which is the catalytic byproduct of a xanthine oxidase (XO) enzyme. In this work, we investigated the inhibition kinetics of XO and identified bioactive compounds from the stem extract fractions of S. littoralis Hassk. using liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). Fractionation was carried out to obtain n-hexane, dichloromethane, and 1-butanol fractions from the water and 70% ethanol extracts. Fraction of 1-butanol from 70% ethanol and water extract displayed potent inhibitors of XO with IC50 value 116.91±3.51 and 137.15±5.00 mg/L, respectively. Lineweaver-Burk plot analysis showed that the 1-butanol fraction from the two extracts inhibited XO competitively. The 1-butanol fraction from the two extracts has been further identified as a bioactive fraction. The majority of the compounds in the two active fractions were phenolics. These findings revealed that the 1-butanol fraction from the two extracts is promising as an antigout treatment in the future.

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滨海鸡血藤抗痛风活性研究。黄嘌呤氧化酶提取物:代谢谱及抑制动力学

滨海鸡血藤。(S. littoralis Hassk.)是印度尼西亚的一种本地植物，在传统的大雅医学中被广泛用于治疗非传染性退行性疾病。其中一种疾病，被称为痛风，是由血液中过量的尿酸引起的，尿酸是黄嘌呤氧化酶(XO)的催化副产物。在这项工作中，我们研究了XO的抑制动力学和鉴定的生物活性化合物，从海荆芥茎提取部分。采用液相色谱-质谱/质谱(LC-MS/MS)。从水和70%乙醇提取物中分馏得到正己烷、二氯甲烷和1-丁醇馏分。70%乙醇和水提取物的1-丁醇组分对XO有较强的抑制作用，IC50值分别为116.91±3.51和137.15±5.00 mg/L。Lineweaver-Burk图分析表明，两种提取物的1-丁醇组分对XO具有竞争性抑制作用。从这两种提取物中提取的1-丁醇部分已被进一步鉴定为生物活性部分。两种活性组分中以酚类化合物居多。这些发现表明，从这两种提取物中提取的1-丁醇部分有希望在未来作为一种抗痛风治疗。

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来源期刊

HAYATI Journal of Biosciences Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)

CiteScore

1.10

自引率

0.00%

发文量

审稿时长

24 weeks

期刊介绍： HAYATI Journal of Biosciences (HAYATI J Biosci) is an international peer-reviewed and open access journal that publishes significant and important research from all area of biosciences fields such as biodiversity, biosystematics, ecology, physiology, behavior, genetics and biotechnology. All life forms, ranging from microbes, fungi, plants, animals, and human, including virus, are covered by HAYATI J Biosci. HAYATI J Biosci published by Department of Biology, Bogor Agricultural University, Indonesia and the Indonesian Society for Biology. We accept submission from all over the world. Our Editorial Board members are prominent and active international researchers in biosciences fields who ensure efficient, fair, and constructive peer-review process. All accepted articles will be published on payment of an article-processing charge, and will be freely available to all readers with worldwide visibility and coverage.