Dark septate endophyte inoculation improved Pb phytoremediation of Jatropha curcas and Reutealis trisperma on gold mine tailings

Abstract

AbstractThis study investigated the symbiotic potential of Jatropha curcas and Reutealis trisperma with two isolates of dark septate endophyte (DSE) fungi (Cladosporium sp. and Rhyzopycnis vagum) grown on gold mine tailings media based on their ability to remediate Pb and their morphological, physiological, and anatomical changes. The targeted plants were grown for 12 weeks on different media contained mixed soil and compost, gold mine tailings, and tailing with additional charcoal. The result showed that DSE inoculation increased the height of J. curcas up to 24.1% in contaminated soil compared to control treatment without DSE. All DSE fungi were able to colonize both root plant even under gold mine tailing treatment. DSE inoculation increased chlorophyll content of both plants ranging from 11% to 33% compared to the plants without DSE. Jatropha curcas inoculated with DSE had the potential to be used as phytoremediation plant with a phytoextraction mechanism indicated by higher translocation factor (TF) value. Meanwhile, the R. trisperma was able to be used for phytoremediation categorized as phytostabilization due to lower TF values. The results of this study highlighted the possible role of the DSE symbiotic with J. curcas and R. trisperma to improve the efficiency of phytoremediation in contaminated lands.Keywords: Dark septate endophytesJatropha curcasmine tailingphytoremediationReutealis trisperma AcknowledgmentsAuthors also would like to say thank you to the Indonesian Soil Research Institute for providing the dark septate endophyte inoculant. Finally, we thanks to PT Aneka Tambang (ANTAM) Pongkor, Bogor Indonesia, for their support to use gold mine tailings for this research.Disclosure statementNo potential conflict of interest was reported by the author(s).Additional informationFundingThe authors received the support from the Ministry of Education and Culture of the Republic of Indonesia for funding research under the 2019 Penelitian Dasar Unggulan Perguruan Tinggi (PDUPT) with the Grant number of 1/E1/KP.PTNBH/2021 on 8 March 2021.