[Application of high resolution liquid chromatography to the test of bile acid tolerance].

Giornale di clinica medica Pub Date : 1990-01-01
F Marotta, L Hashimoto, P Safran
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Abstract

In the present paper we have evaluated the accuracy of a fully automated liquid-chromatographic method to study the variations of serum bile acid concentration after oral administration of 500 mg of chenodeoxycholic as a bile acid tolerance test. The study population consisted of 11 subjects with liver cirrhosis (L.C.), 6 with chronic active hepatitis (C.A.H.) and of 15 healthy volunteers, as a control. A clear linear correlation was observed between the integrated peak area and the concentration of each bile acid. Bile acids were detected at a minimum concentration of 5 ng. Intra-assay variation, based on 10 consecutive determinations, was limited to a range of 0.42% and 3.23%. Compared to control group, L.C. and C.A.H. patients showed significantly higher levels of total bile acids and of CDCA. Significative was also the increase of glycine- and taurine-conjugates as well as the decrease of the ratio between the two. The present method, fully automated and using a low cost enzymatic reagent, has yielded an accurate analysis of bile acid fractions on a minute volume of serum in a short examination time.

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高分辨液相色谱法在胆汁酸耐受性检测中的应用
在本文中,我们评估了全自动液相色谱法研究口服500毫克鹅去氧胆酸作为胆汁酸耐量试验后血清胆汁酸浓度变化的准确性。研究人群包括11名肝硬化(L.C.)患者,6名慢性活动性肝炎(C.A.H.)患者和15名健康志愿者作为对照。综合峰面积与各胆汁酸浓度呈明显的线性相关。最低浓度为5 ng时检测胆汁酸。基于连续10次测定,测定内变异限制在0.42%和3.23%的范围内。与对照组相比,L.C.和C.A.H.患者的总胆汁酸和CDCA水平明显升高。值得注意的是甘氨酸和牛磺酸缀合物的增加以及两者之间比率的降低。目前的方法是全自动的,使用低成本的酶试剂,在很短的检查时间内对一分钟体积的血清中的胆汁酸组分进行了准确的分析。
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