Evaluation of Antimicrobial Activities of Different Extracts from Phlomis tuberosa, Abutilon fruticosum, and Nepeta transcaucasica

Q4 Biochemistry, Genetics and Molecular Biology Acta Microbiologica Bulgarica Pub Date : 2023-06-01 DOI:10.59393/amb23390211
S. Asnaashari, F. Heshmati-Afshar, N. Amin-Aghdam, P. Asgharian, S. Hallaj-Nezhadi
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Abstract

The use of medicinal plants for the natural treatment of infections has received much attention. Pre¬viously, plants of Nepeta, Phlomis, and Abutilon genera showed remarkable antimicrobial activities. We aimed to evaluate the antimicrobial effects of three Iranian plants including Abutilon fruticosum Guill. & Perr., Nepeta transcaucasica Grossh., and Phlomis tuberosa (L.) Moench. Aerial parts of the plants were extracted by the maceration method using petroleum ether, chloroform, ethyl acetate (EtAc), and methanol solvents. The antimicrobial activity of the extracts against seven Gram-positive strains and three Gram-neg-ative strains was studied via the disk diffusion method. Potent extracts were fractionated and diameters of the inhibition zone (DIZ) and Minimum Inhibitory Concentration (MIC) were determined. The chloroform extract of N. transcaucasica and EtAc extract of A. fruticosum were the most active extracts. Staphylococ¬cus aureus, was the most sensitive strain to N. transcaucasica (DIZ=17 mm, MIC=3.125 mg/ml), whereas S. epidermidis (DIZ=15 mm, MIC=1.56 mg/ml), and Bacillus subtilis (DIZ=13mm, MIC=0.39 mg/ml) were the most sensitive bacteria to A. fruticosum. Fractions of 40% and 60% EtAc/n-hexane of chloroform extract of N. transcaucasica and fractions of 40%-100% of EtAc extract of A. fruticusum revealed the potent activities. B. cereus was “very sensitive” to 60%-100% fractions of chloroform extract of N. trans¬caucasica (DIZ=16–19 mm, MIC=12.5 mg/ml). S. pneumoniae was “extremely” (DIZ=20 mm) sensitive to the 60% Ethyl acetate fraction of A. fruticosum and noticeably, their antimicrobial effects were greater than that of the standard antibiotic. The results support some of the traditional applications of the studied plants.
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结核、槟榔和外高加索荆芥不同提取物的抑菌活性评价
利用药用植物自然治疗感染已受到广泛关注。在此之前,Nepeta属、Phlomis属和Abutilon属植物都表现出了显著的抑菌活性。本研究旨在评价包括Abutilon fruticosum Guill在内的三种伊朗植物的抗菌作用。,穗青葱。外高加索尼佩塔。和结核杆菌(L.)。Moench。用石油醚、氯仿、乙酸乙酯(EtAc)和甲醇溶剂浸渍法提取植物的地上部分。采用纸片扩散法对7株革兰氏阳性菌和3株革兰氏阴性菌的抑菌活性进行了研究。对有效提取物进行分馏,测定其抑菌带直径和最小抑菌浓度。外高加索三氯甲烷提取液和枇杷叶乙酸乙酯提取液活性最强。金黄色葡萄球菌对外高加索葡萄球菌(DIZ=17 mm, MIC=3.125 mg/ml)最敏感,表皮葡萄球菌(DIZ=15 mm, MIC=1.56 mg/ml)和枯草芽孢杆菌(DIZ=13mm, MIC=0.39 mg/ml)对果实葡萄球菌最敏感。外高加索三氯甲烷提取液中40%和60%的乙酸乙酯/正己烷含量和40% ~ 100%的木香乙酸乙酯含量显示出较强的活性。蜡样芽孢杆菌对60% ~ 100%的泛高加索白参氯仿提取物(DIZ=16 ~ 19 mm, MIC=12.5 mg/ml)“非常敏感”。肺炎链球菌对枸杞60%乙酸乙酯部位“极”(DIZ=20 mm)敏感,抑菌效果明显优于标准抗生素。研究结果支持了所研究植物的一些传统应用。
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来源期刊
Acta Microbiologica Bulgarica
Acta Microbiologica Bulgarica Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
0.40
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0
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