Evaluation of catechin, lupeol, and betulinic acid as markers for the chromatographic quality Control of Albizia coriaria raw materials; an experimental study
{"title":"Evaluation of catechin, lupeol, and betulinic acid as markers for the chromatographic quality Control of Albizia coriaria raw materials; an experimental study","authors":"Kaggwa Bruhan, Ireeta Munanura Edson, Kyeyune Henry, Anywar Godwin, Okella Hedmon, Olusoji Ajayi Clement, Wangalwa Raphael, Mulangwa John, Duncan Sesaazi Crispin, K. Bagoloire Lynn, Umba Tolo Casim, Fadhiru Kamba Pakoyo, Engeu Ogwang Patrick","doi":"10.5897/jmpr2022.7285","DOIUrl":null,"url":null,"abstract":"Albizia coriaria stembark is among the most common raw materials used to manufacture herbal products in Uganda. While the plant material is sourced from the wild, there are neither monographs nor chemical analytical methods for its standardisation. In addition, good cultivation, harvesting, and manufacturing practices are not mandatory. This leads to inconsistent quality of raw materials and products. This work developed TLC and HPLC methods and evaluated the suitability of lupeol, catechin, and betulinic acid markers for standardization of stembark from A. coriaria. Samples were extracted by decoction, maceration and soxhlet reflux; in water, ethanol and ethyl acetate. Catechin, lupeol and betulinic acid markers were found in all batches of A. coriaria from different agroecological zones, at concentrations higher than 6mg/g. Ethyl acetate fractions gave the highest marker quantities, while decoction gave the lowest. The optimised TLC fingerprint conditions were solvent system: 4, 2, 1 and 1 parts of n-hexane, ethyl acetate, dichloromethane, and methanol, respectively; stationary phase; TLC glass plates, 10 cm × 20 cm, coated with fluorescent 60Å silica gel matrices and vanillin / sulfuric acid spray reagent. The resulting fingerprints comprised of twelve principal peaks. The optimized conditions of the HPLC fingerprint and quantitation method were: C18 column (250 × 4.6 mm x 5 µm), solvent system; acetonitrile (99.99%) and 1% trifluoroacetic acid (0.01%); flow rate, 1mL/min; column temperature, 25°C; and UV/visible detector 205 nm. The common HPLC fingerprints had correlation coefficient >0.99, while quantitative methods were accurate (>97% recovery) and reproducible (RSD < 0.38%). Lupeol and betulinic acid are suitable markers for the quality control of Albizia coriaria raw materials. decoctions are better controlled by catechin. Key words: Markers, fingerprints, Albizia coriaria, betulinic acid, lupeol, quality control, standardisation","PeriodicalId":16387,"journal":{"name":"Journal of Medicinal Plants Research","volume":"46 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Medicinal Plants Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5897/jmpr2022.7285","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
Albizia coriaria stembark is among the most common raw materials used to manufacture herbal products in Uganda. While the plant material is sourced from the wild, there are neither monographs nor chemical analytical methods for its standardisation. In addition, good cultivation, harvesting, and manufacturing practices are not mandatory. This leads to inconsistent quality of raw materials and products. This work developed TLC and HPLC methods and evaluated the suitability of lupeol, catechin, and betulinic acid markers for standardization of stembark from A. coriaria. Samples were extracted by decoction, maceration and soxhlet reflux; in water, ethanol and ethyl acetate. Catechin, lupeol and betulinic acid markers were found in all batches of A. coriaria from different agroecological zones, at concentrations higher than 6mg/g. Ethyl acetate fractions gave the highest marker quantities, while decoction gave the lowest. The optimised TLC fingerprint conditions were solvent system: 4, 2, 1 and 1 parts of n-hexane, ethyl acetate, dichloromethane, and methanol, respectively; stationary phase; TLC glass plates, 10 cm × 20 cm, coated with fluorescent 60Å silica gel matrices and vanillin / sulfuric acid spray reagent. The resulting fingerprints comprised of twelve principal peaks. The optimized conditions of the HPLC fingerprint and quantitation method were: C18 column (250 × 4.6 mm x 5 µm), solvent system; acetonitrile (99.99%) and 1% trifluoroacetic acid (0.01%); flow rate, 1mL/min; column temperature, 25°C; and UV/visible detector 205 nm. The common HPLC fingerprints had correlation coefficient >0.99, while quantitative methods were accurate (>97% recovery) and reproducible (RSD < 0.38%). Lupeol and betulinic acid are suitable markers for the quality control of Albizia coriaria raw materials. decoctions are better controlled by catechin. Key words: Markers, fingerprints, Albizia coriaria, betulinic acid, lupeol, quality control, standardisation
在乌干达，马鞭草是制造草药产品最常用的原料之一。虽然植物材料来源于野外，但既没有专著，也没有化学分析方法来标准化。此外，良好的种植、收获和生产实践并不是强制性的。这导致原材料和产品质量不一致。建立了薄层色谱(TLC)和高效液相色谱(HPLC)方法，并评价了鹿皮醇、儿茶素和白桦酸标记物在马桑叶叶标准中的适用性。采用煎煮法、浸渍法和索氏反流法提取样品;在水中，乙醇和乙酸乙酯。不同农业生态区各批次马桑中均发现儿茶素、鹿皮酚和白桦酸标记物，浓度均大于6mg/g。乙酸乙酯组分的标记量最高，煎剂的标记量最低。优选的TLC指纹图谱条件为溶剂体系:正己烷、乙酸乙酯、二氯甲烷、甲醇分别为4、2、1、1份;固定相;TLC玻璃板，10厘米&倍;20厘米，涂有荧光60Å硅胶基质和香兰素/硫酸喷雾试剂。所得指纹图谱由12个主峰组成。HPLC指纹图谱及定量方法的优化条件为:C18柱(250次);4.6 mm x 5 &微米)，溶剂系统;乙腈(99.99%)和1%三氟乙酸(0.01%);流量，1mL/min;柱温，25℃;紫外/可见光检测器205 nm。常用HPLC指纹图谱的相关系数为0.99，定量方法准确(回收率为97%)，重现性好(RSD为97%)。0.38%)。木犀皮醇和白桦酸是合欢原料质量控制的适宜标记物。儿茶素能更好地控制煎药。关键词:标记物，指纹图谱，木犀草，白桦酸，鹿皮醇，质量控制，标准化
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