Urinary mitochondrial DNA may be useful in diagnosing early diabetic nephropathy

Abstract

The present study aimed to determine whether urinary mitochondrial (mt)DNA could be combined as a non‑invasive biomarker with other clinical findings of kidney injury to help diagnose early diabetic nephropathy (DN). A total of 165 patients with type 2 diabetes mellitus (T2DM) were enrolled in the present study and the mtDNA levels in urine were measured using quantitative PCR. The diagnostic value of urinary mtDNA levels in patients with T2DM was compared using estimated glomerular filtration rate (eGFR) or albumin‑to‑creatinine ratio staging. Spearman correlation analysis was used to analyze the correlation between urinary mtDNA and other clinical findings. Correlation factors for early DN were assessed using univariate logistic regression analysis. Urinary leukocyte and glucose levels do not interfere with urinary mtDNA levels. In patients with T2DM, the level of urinary mtDNA increases in the early stages of kidney injury and further increases with the severity of kidney injury. Urinary mtDNA levels in patients with eGFR 60‑90 ml/min/1.73 m² were higher than that in patients with eGFR >90 ml/min/1.73 m². The levels of urinary mt89DNA and mt349DNA were negatively correlated with the eGFR level (ρ=‑0.437; P<0.001; ρ=‑0.390; P<0.001) and positively correlated with the level of cystatin C (ρ=0.177; P=0.025; ρ=0.144; P=0.070). Urinary mtDNA is positively correlated with early DN occurrence [odds ratio (OR), 1.330; 95% confidence interval (CI), 1.175‑1.507; P<0.001; OR, 1.328; 95% CI, 1.156‑1.525; P<0.001]. In conclusion, urinary mtDNA combined with other clinical indicators of kidney injury may help the diagnosis of early DN.