{"title":"HPLC-pharmaceutical Analysis of Lantibiotic Nisin in the Industrial Samples Including Expired Sample","authors":"Artur Martynov, Oksana Knysh","doi":"10.17721/moca.2023.87-91","DOIUrl":null,"url":null,"abstract":"Nisin is the most well-known representative of subtype A1 lantibiotics. This small (< 5 kDa) peptide ribosomally produced by Gram-positive bacteria belonging to Lactococcus, Streptococcus, Staphylococcus and Blautia species. Nisin exhibits antibacterial activity against a broad range of Gram-positive bacteria and may be effective against Gram-negative pathogens. For analytical semi-preparative purposes, gradient HPLC is often used in the acetonitrile concentration range from 20 to 30 % (solvent B) and a retention time of 20 to 50 min. In this study the optimal conditions for the analysis of nisin by RP-HPLC were determined: a gradient from 23 to 28 % acetonitrile (buffer B) when used as solvents: buffer A: [4 M LiClO4 – 0.1 M HClO4 ] : H2O=1:19; and buffer B: 100 % CH3CN with a retention time up to 12 min. Differences between the chromatographic profiles of expired and non-expired nisin samples have been identified. The expired nisin sample differs from the non-expired samples by the presence of asymmetric nisin A / Z peaks with significant degradation of the nisin A peak. The results of the study indicate the possibility using RP-HPLC for checking the quality and shelf life of commercial nisin samples without the need for additional purification","PeriodicalId":18626,"journal":{"name":"Methods and Objects of Chemical Analysis","volume":null,"pages":null},"PeriodicalIF":0.7000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Methods and Objects of Chemical Analysis","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17721/moca.2023.87-91","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Nisin is the most well-known representative of subtype A1 lantibiotics. This small (< 5 kDa) peptide ribosomally produced by Gram-positive bacteria belonging to Lactococcus, Streptococcus, Staphylococcus and Blautia species. Nisin exhibits antibacterial activity against a broad range of Gram-positive bacteria and may be effective against Gram-negative pathogens. For analytical semi-preparative purposes, gradient HPLC is often used in the acetonitrile concentration range from 20 to 30 % (solvent B) and a retention time of 20 to 50 min. In this study the optimal conditions for the analysis of nisin by RP-HPLC were determined: a gradient from 23 to 28 % acetonitrile (buffer B) when used as solvents: buffer A: [4 M LiClO4 – 0.1 M HClO4 ] : H2O=1:19; and buffer B: 100 % CH3CN with a retention time up to 12 min. Differences between the chromatographic profiles of expired and non-expired nisin samples have been identified. The expired nisin sample differs from the non-expired samples by the presence of asymmetric nisin A / Z peaks with significant degradation of the nisin A peak. The results of the study indicate the possibility using RP-HPLC for checking the quality and shelf life of commercial nisin samples without the need for additional purification
期刊介绍:
The journal "Methods and objects of chemical analysis" is peer-review journal and publishes original articles of theoretical and experimental analysis on topical issues and bio-analytical chemistry, chemical and pharmaceutical analysis, as well as chemical metrology. Submitted works shall cover the results of completed studies and shall make scientific contributions to the relevant area of expertise. The journal publishes review articles, research articles and articles related to latest developments of analytical instrumentations.