HPLC-pharmaceutical Analysis of Lantibiotic Nisin in the Industrial Samples Including Expired Sample

IF 0.7 Q4 CHEMISTRY, ANALYTICAL Methods and Objects of Chemical Analysis Pub Date : 2023-01-01 DOI:10.17721/moca.2023.87-91
Artur Martynov, Oksana Knysh
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Abstract

Nisin is the most well-known representative of subtype A1 lantibiotics. This small (< 5 kDa) peptide ribosomally produced by Gram-positive bacteria belonging to Lactococcus, Streptococcus, Staphylococcus and Blautia species. Nisin exhibits antibacterial activity against a broad range of Gram-positive bacteria and may be effective against Gram-negative pathogens. For analytical semi-preparative purposes, gradient HPLC is often used in the acetonitrile concentration range from 20 to 30 % (solvent B) and a retention time of 20 to 50 min. In this study the optimal conditions for the analysis of nisin by RP-HPLC were determined: a gradient from 23 to 28 % acetonitrile (buffer B) when used as solvents: buffer A: [4 M LiClO4 – 0.1 M HClO4 ] : H2O=1:19; and buffer B: 100 % CH3CN with a retention time up to 12 min. Differences between the chromatographic profiles of expired and non-expired nisin samples have been identified. The expired nisin sample differs from the non-expired samples by the presence of asymmetric nisin A / Z peaks with significant degradation of the nisin A peak. The results of the study indicate the possibility using RP-HPLC for checking the quality and shelf life of commercial nisin samples without the need for additional purification
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包括过期样品在内的工业样品中抗生素Nisin的hplc -药学分析
Nisin是A1亚型抗生素中最著名的代表。这个小的(<5 kDa)肽核糖体产生的革兰氏阳性菌属于乳球菌,链球菌,葡萄球菌和蓝菌属。Nisin对多种革兰氏阳性细菌具有抗菌活性,可能对革兰氏阴性病原体有效。在半制备分析中,梯度高效液相色谱常用于乙腈浓度为20% ~ 30%(溶剂B),保留时间为20 ~ 50 min的条件下。本研究确定了反相高效液相色谱分析nisin的最佳条件:当溶剂为23% ~ 28%乙腈(缓冲液B)时,缓冲液a: [4 M LiClO4 - 0.1 M HClO4]: H2O=1:19;缓冲液B: 100% CH3CN,保留时间长达12分钟。过期和未过期nisin样品的色谱图谱存在差异。过期的nisin样品与未过期样品的不同之处在于存在不对称的nisin A / Z峰,nisin A峰明显降解。该研究结果表明,使用反相高效液相色谱法检查商品nisin样品的质量和保质期的可能性,而无需额外的纯化
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来源期刊
CiteScore
1.00
自引率
14.30%
发文量
12
期刊介绍: The journal "Methods and objects of chemical analysis" is peer-review journal and publishes original articles of theoretical and experimental analysis on topical issues and bio-analytical chemistry, chemical and pharmaceutical analysis, as well as chemical metrology. Submitted works shall cover the results of completed studies and shall make scientific contributions to the relevant area of expertise. The journal publishes review articles, research articles and articles related to latest developments of analytical instrumentations.
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