Development and Evaluation of Real-Time Polymerase Chain Reaction (PCR) to Authenticate Sablefish ( Anoplopoma fimbria ) in Commercial Seafood Products

Abstract

ABSTRACTBy comparing the cytochrome b fragment sequences of sablefish and 16 varieties susceptible to adulteration, a real-time polymerase chain reaction (PCR) method was established to detect sablefish-derived components. The PCR method was used to analyze commercial products. The method developed was highly specific without any cross-reaction in the other 16 species and with a limit of detection for deoxyribonucleic acid (DNA) of 0.002 ng µL−1 for sablefish. In addition, the mixed muscle tissues were detected at as low as 0.01 g kg−1. The applicability of the PCR method to 50 commercial prepackaged products revealed the accuracy of this method.KEYWORDS: Sablefishreal-time polymerase chain reactionauthenticationcytochrome b gene Disclosure statementThe authors declare no competing financial interest.Data availability statementThe datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.Additional informationFundingThis work was supported by the National Key Research and Development Program of China under Grant [number 2019YFC1604702]; National Key Research and Development Program of China under Grant [number 2016YFF0201805]; Central Public-interest Scientific Institution Basal Research Fund, CAFS under Grant [2020TD71]; and Central Public-interest Scientific Institution Basal Research Fund, CAFS under Grant [2021JC01].