{"title":"Evaluation of bromelain and temozolomide synergistic combination in human glioblastoma cells","authors":"Megha Gautam, Reema Gabrani","doi":"10.1007/s13596-023-00717-y","DOIUrl":null,"url":null,"abstract":"<div><p>The development of effective therapy is required for glioblastoma multiforme (GBM) patients because of the resistance action to temozolomide (TMZ). Bromelain (BRO) is a plant-derived therapeutic used against inflammation. The objective of this study is to appraise the effect of the BRO + TMZ combination on U87MG and LN229 cells and investigate its mechanism. The BRO + TMZ treatment resulted in synergistic effects that restricted cell growth and inhibited the colony formation of GBM cells. The combined treatment led to the initiation of early apoptosis resulting in the overexpression of BAX/caspase-3 (CASP3) and decreased transcript level of BCL-2. The BRO + TMZ induced the arrest in G0/G1 phase and decreased the migration mediated by the dysregulation of MMP-2 and MMP-9. The BRO and TMZ combination treatment was observed to be more effective than the individual compound to inhibit cell growth in human GBM cell lines.</p></div>","PeriodicalId":7613,"journal":{"name":"Advances in Traditional Medicine","volume":"24 2","pages":"507 - 518"},"PeriodicalIF":1.8000,"publicationDate":"2023-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advances in Traditional Medicine","FirstCategoryId":"1085","ListUrlMain":"https://link.springer.com/article/10.1007/s13596-023-00717-y","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
引用次数: 0
Abstract
The development of effective therapy is required for glioblastoma multiforme (GBM) patients because of the resistance action to temozolomide (TMZ). Bromelain (BRO) is a plant-derived therapeutic used against inflammation. The objective of this study is to appraise the effect of the BRO + TMZ combination on U87MG and LN229 cells and investigate its mechanism. The BRO + TMZ treatment resulted in synergistic effects that restricted cell growth and inhibited the colony formation of GBM cells. The combined treatment led to the initiation of early apoptosis resulting in the overexpression of BAX/caspase-3 (CASP3) and decreased transcript level of BCL-2. The BRO + TMZ induced the arrest in G0/G1 phase and decreased the migration mediated by the dysregulation of MMP-2 and MMP-9. The BRO and TMZ combination treatment was observed to be more effective than the individual compound to inhibit cell growth in human GBM cell lines.
期刊介绍：
Advances in Traditional Medicine (ADTM) is an international and peer-reviewed journal and publishes a variety of articles including original researches, reviews, short communications, and case-reports. ADTM aims to bridging the gap between Traditional knowledge and medical advances. The journal focuses on publishing valid, relevant, and rigorous experimental research and clinical applications of Traditidnal Medicine as well as medical classics. At the same time, the journal is devoted to communication among basic researcher and medical clinician interested in the advancement of Traditional Medicine. Topics covered by the journal are: Medical Classics & History; Biomedical Research; Pharmacology & Toxicology of Natural Products; Acupuncture & Moxibustion; Sasang Constitutional Medicine; Diagnostics and Instrumental Development; Clinical Research. ADTM is published four times yearly. The publication date of this journal is 30th March, June, September, and December.
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