Additive Effects of High Fructose Corn Syrup (HFCS) in Experimental Oral Carcinogenesis

IF 0.7 Q4 PHARMACOLOGY & PHARMACY Pharmacognosy Research Pub Date : 2023-11-08 DOI:10.5530/pres.15.4.087
Kavitha Kalimuthu, Sindhu Ganapathy, Balamurugan Elumalai, Asha Kumarasamypillai Radha Thayammal, Veeran Veeravarmal, Vijayalakshmi Annamalai
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Abstract

Pharmacognosy Research,2023,15,4,819-830.DOI:10.5530/pres.15.4.087Published:October 2023Type:Original ArticleAuthors:Kavitha Kalimuthu, Sindhu Ganapathy, Balamurugan Elumalai, Asha Kumarasamypillai Radha Thayammal, Veeran Veeravarmal, and Vijayalakshmi Annamalai Author(s) affiliations:Kavitha Kalimuthu1, Sindhu Ganapathy1,2,*, Balamurugan Elumalai1, Asha Kumarasamypillai Radha Thayammal3, Veeran Veeravarmal4, Vijayalakshmi Annamalai5 1Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar, Chidambaram, Tamil Nadu, INDIA. 2Department of Biochemistry, Government Arts College (Autonomous), Kumbakonam, Tamil Nadu, INDIA. 3Department of Biochemistry, Government Arts College, Paramakudi, Tamil Nadu, INDIA. 4Department of Oral and Maxillofacial Pathology, Rajah Muthiah Dental College and Hospital, Annamalai Nagar, Tamil Nadu, INDIA. 5Plant Head, Galileovasan Offshore and Research and Development Private Limited, Nagapattinam, Tamil Nadu, INDIA. Abstract:Introduction: Nowadays, fructose uses dramatically increased in form of High-Fructose Corn Syrup (HFCS) found in juices and packed food. Sustained fructose utilization is detrimental to long-term human health. Objectives: To assess the additive effects of HFCS during 7,12-Dimethylbenz(a)Anthracene (DMBA)-induced Hamster Buccal Pouch Carcinogenesis (HBPCs) model. Materials and Methods: The animals were separated into eight groups: Group I; vehicle control; Group II (0.5% DMBA); Group III and IV (HFCS 8% and 25%); Group V (Sucrose 10%); Group VI and VII (0.5% DMBA+HFCS 8 and 25%) and VIII group (0.5% DMBA+Sucrose 10%) respectively for 14 weeks. After the 14th week of treatment; the tumor morphology, buccal histopathology, and biochemical markers were measured and compared with carcinogenic control as well as vehicle control. Observations and Results: The buccal pouch of golden Syrian hamsters developed well-differentiated squamous cell carcinoma after getting topical applications of 0.5% DMBA in liquid paraffin three times a week for 14 weeks. Although DMBA treatment alone caused 100% tumor development in hamsters, drinking water administration of HFCS at a concentration of 25%/kg body weight (b.w.) to DMBA-treated hamster greatly accelerated the development of oral tumors. Additionally, during DMBA-induced oral carcinogenesis, HFCS moderatingly increased the lipid peroxidation by-products, decreased the status of enzymatic and non-enzymatic antioxidants, modulated the levels of phase I and phase II detoxification agents, and favored the excretion of carcinogenic metabolite. Conclusion: The present study concludes that the additive effect of HFCS relies on its altered peroxidative and antioxidant function as well as effects on phase I and II detoxification enzymes during DMBA-induced hamster buccal pouch carcinogenesis. Taken together the current study described that HFCS induced oral tumour development. From this study we suggested HFCS usage to be curtailed. Keywords:Antioxidants, Detoxification enzymes, DMBA, Hamsters, HFCS, Lipid peroxidation, Oral cancerView:PDF (704.72 KB)
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高果糖玉米糖浆(HFCS)在实验性口腔癌中的加性作用
Pharmacognosy Research,2023,15,4,819-830.DOI:10.5530/pres.15.4.087发表时间:2023 年 10 月类型:原创文章作者:Kavitha Kalimuthu, Sindhu Ganapathy, Balamurugan Elumalai, Asha Kumarasamypillai Radha Thayammal, Veeran Veeravarmal, and Vijayalakshmi Annamalai作者单位:中国科学院上海生命科学研究院:Kavitha Kalimuthu1, Sindhu Ganapathy1,2,*, Balamurugan Elumalai1, Asha Kumarasamypillai Radha Thayammal3, Veeran Veeravarmal4, Vijayalakshmi Annamalai5 1Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar, Chidambaram, Tamil Nadu, INDIA.2Department of Biochemistry, Government Arts College (Autonomous), Kumbakonam, Tamil Nadu, INDIA.3Department of Biochemistry, Government Arts College, Paramakudi, Tamil Nadu, INDIA.4Department of Oral and Maxillofacial Pathology, Rajah Muthiah Dental College and Hospital, Annamalai Nagar, Tamil Nadu, INDIA.5 印度泰米尔纳德邦纳加帕蒂纳姆伽利略瓦桑近海和研发私人有限公司工厂主管。摘要:引言:如今,果汁和包装食品中的高果糖玉米糖浆(HFCS)形式的果糖用量急剧增加。持续使用果糖不利于人类的长期健康。目标:评估高果糖糖浆在 7,12-二甲基苯并(a)蒽(DMBA)诱导的仓鼠颊囊致癌模型(HBPCs)中的添加效应。材料与方法:动物分为八组:第一组;药物对照;第二组(0.5% DMBA);第三组和第四组(HFCS 8%和 25%);第五组(蔗糖 10%);第六组和第七组(0.5% DMBA+HFCS 8%和 25%)以及第八组(0.5% DMBA+蔗 糖 10%),分别治疗 14 周。治疗第 14 周后,测量肿瘤形态、口腔组织病理学和生化指标,并与致癌物对照组和药物对照组进行比较。观察结果每周三次在液体石蜡中外敷 0.5% DMBA,连续 14 周后,金色叙利亚仓鼠的颊囊出现了分化良好的鳞状细胞癌。虽然单独使用 DMBA 会导致仓鼠 100% 肿瘤发生,但在饮用水中添加浓度为 25%/kg 体重(b.w.)的 HFCS 会大大加速口腔肿瘤的发展。此外,在 DMBA 诱导的口腔癌发生过程中,HFCS 会适度增加脂质过氧化副产物,降低酶和非酶抗氧化剂的状态,调节 I 期和 II 期解毒剂的水平,并促进致癌代谢物的排泄。结论本研究的结论是,在 DMBA 诱导的仓鼠颊囊癌变过程中,HFCS 的添加效应依赖于其过氧化和抗氧化功能的改变,以及对 I 期和 II 期解毒酶的影响。总之,本研究描述了 HFCS 诱导口腔肿瘤发生的情况。通过这项研究,我们建议减少使用 HFCS。关键词:抗氧化剂 解毒酶 DMBA 仓鼠 HFCS 脂质过氧化 口腔癌查看:PDF (704.72 KB)
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Pharmacognosy Research
Pharmacognosy Research PHARMACOLOGY & PHARMACY-
自引率
14.30%
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期刊介绍: Pharmacognosy Research [ISSN: Print -0976-4836, Online - 0974-8490] [http://www.phcogres.com], Quarterly a publication of Phcog.Net is published by Wolters Kluwer - Medknow Publications. It provides peer-reviewed original research articles from the field of Natural Products. The journal serves an international audience of scientists and researchers in a variety of research and academia by quickly disseminating research findings related to Medicinal Plants and Natural Products. It is a peer reviewed journal aiming to publish high quality original research articles, methods, techniques and evaluation reports, critical reviews, short communications, commentaries and editorials of all aspects of medicinal plant research. The journal is aimed at a broad readership, publishing articles on all aspects of pharmacognosy, and related fields. The journal aims to increase understanding of pharmacognosy as well as to direct and foster further research through the dissemination of scientific information by the publication of manuscripts. The submissions of original contributions in all areas of pharmacognosy are welcome.
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