Serum Metallomics Reveals Insights into the Associations of Elements with the Progression of Preleukemic Diseases Towards Acute Leukemia

IF 2.5 Q3 BIOCHEMICAL RESEARCH METHODS Biology Methods and Protocols Pub Date : 2023-11-01 DOI:10.1093/biomethods/bpad027
Amna Jabbar Siddiqui, Noman Khan, Kauser Fatima, Sabiha Farooq, Muhammad Ramzan, Hesham R El-Seedi, Jalal Uddin, Abdullatif Bin Muhsinah, Syed Ghulam Musharraf
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Abstract

Abstract Context Acute leukemia (AL) is a critical neoplasm of white blood cells with two main subtypes: acute lymphoblastic leukemia (ALL) and acute myeloid leukemia (AML). Objective This study is focused on understanding the association of the preleukemic disease aplastic anemia (APA) with ALL and AML at metallomic level, using healthy subject as a control. Materials and methods In this study, a validated and efficient ICP-MS/MS-based workflow was employed to profile a total of 13 metallomic features. The study encompassed 41 patients with AML, 62 patients with ALL, 46 patients with APA, and 55 age-matched healthy controls. The metallomic features consisted of 8 essential elements (Ca, Co, Cu, Fe, Mg, Mn, Se, and Zn) and 5 non-essential/toxic elements (Ag, Cd, Cr, Ni, and Pb). Results Six out of the thirteen elements were found to be substantially different (p < 0.05) using absolute concentrations between serum samples of acute leukemia (ALL and AML) and preleukemia (APA) patients in comparison with healthy subjects. Elements including magnesium, calcium, iron, copper and zinc were up-regulated and only one element (chromium) was down-regulated in serum samples of disease when compared with healthy subjects. Discussion Through the utilization of both univariate tests and multivariate classification modeling, it was determined that chromium exhibited a progressive behavior among the studied elements. Specifically, chromium displayed a sequential up-regulation from healthy individuals to preleukemic disease (APA), and ultimately in patients diagnosed with ALL. Conclusion Overall, metallomic-based biomarkers may have utility to predict the association of APA with ALL.
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血清金属组学揭示了与白血病前期疾病向急性白血病进展相关的元素
摘要背景急性白血病(Acute leukemia, AL)是一种重要的白细胞肿瘤,主要有两种亚型:急性淋巴细胞白血病(Acute lymphoblastic leukemia, ALL)和急性髓系白血病(Acute myeloid leukemia, AML)。目的以健康受试者为对照,从金属学水平探讨白血病前再障(APA)与ALL和AML的关系。材料和方法在本研究中,采用了一种经过验证且高效的基于ICP-MS/ ms的工作流程来分析总共13个金属学特征。该研究包括41名AML患者,62名ALL患者,46名APA患者和55名年龄匹配的健康对照。金属特征包括8种必需元素(Ca、Co、Cu、Fe、Mg、Mn、Se和Zn)和5种非必需/有毒元素(Ag、Cd、Cr、Ni和Pb)。结果发现13个元素中有6个存在显著差异(p <0.05),用急性白血病(ALL和AML)和白血病前期(APA)患者血清样本的绝对浓度与健康受试者比较。与健康受试者相比,疾病血清样品中的镁、钙、铁、铜和锌元素上调,只有一种元素(铬)下调。通过单变量检验和多变量分类模型的利用,确定了铬在研究元素中表现出递进行为。具体来说,铬从健康个体到白血病前期疾病(APA),并最终在ALL患者中表现出顺序上调。结论基于金属组学的生物标志物在预测APA与ALL的相关性方面可能具有实用价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biology Methods and Protocols
Biology Methods and Protocols Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
CiteScore
3.80
自引率
2.80%
发文量
28
审稿时长
19 weeks
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