Development of a Botanical Formulation Using <em>Cinnamomum verum</em> and <em>Pongamia pinnata</em> Leaves for Suppression of Nitrification in Soil

A. M. Dias, W. S. Dandeniya
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Abstract

There is a growing interest in controlling nitrification by using botanical nitrification inhibitors to enhance the nitrogen (N) fertilizer use efficiency. The main objective of this study was to develop a botanical formulation (BF) with Cinnamomum verum (cinnamon) and Pongamia pinnata (karanda) leaves to suppress the nitrification rate of two soils (Red Yellow Podzolic (RYP) and Reddish-Brown Earth (RBE)) commonly used for vegetable cultivation in Sri Lanka. A BF was prepared using dried and powdered leaves of cinnamon and karanda, biochar and corn flour. The two soils were treated with BF at three rates (0, 5 and 10%, w/w) and the potential nitrification rate (PNR) was determined using the shaken slurry method in a laboratory incubation. The obtained data were analysed by Statistical Analysis Software (SAS) adopting a two factor factorial design. The BF application significantly delayed (P<0.05) nitrate formation in both soils. The RBE soil had significantly lesser (P<0.05) PNR compared to RYP soil. Application of 5 and 10% BF, significantly reduced PNR in both soils at 4 h of incubation. However, only a 10% BF application rate remained effective in suppressing PNR at 21 h of incubation. Results indicate the possibility of using dried, powdered leaves of cinnamon and karanda for suppressing nitrification in soil. Further studies are required to investigate the effect of developed BF on soil biological properties.
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肉桂植物制剂的研制<em>和<em>Pongamia pinnata</em>抑制土壤硝化作用的叶片
利用植物性硝化抑制剂控制硝化作用以提高氮肥利用效率的研究日益受到关注。本研究的主要目的是开发一种植物制剂(BF),以肉桂(肉桂)和凤头蓬(卡兰达)叶为原料,抑制斯里兰卡蔬菜种植常用的两种土壤(红黄灰化土(RYP)和红棕土(RBE))的硝化速率。以肉桂叶、卡兰达叶、生物炭和玉米粉为原料,制备了一种生物质燃料。分别以0、5%和10% (w/w)三种浓度的BF处理两种土壤,并在实验室培养中采用摇浆法测定潜在硝化速率(PNR)。所得资料采用SAS统计分析软件进行分析,采用双因素析因设计。施用BF显著延缓了两种土壤中硝酸盐的形成(P<0.05)。RBE土壤的PNR显著低于RYP土壤(p < 0.05)。在培养4 h时,施用5%和10%的BF显著降低了两种土壤的PNR。然而,在培养21 h时,只有10%的BF施用量仍能有效抑制PNR。结果表明,肉桂和卡兰达的干燥、粉末状叶可以抑制土壤中的硝化作用。发酵后的BF对土壤生物学特性的影响有待进一步研究。
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