Lighting up plants with near-infrared fluorescence probes

IF 10.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Science China Chemistry Pub Date : 2023-10-23 DOI:10.1007/s11426-023-1815-9

Yida Pang, Mengjiao Lu, Hyeonji Rha, Wenchao Yang, Amit Sharma, Yao Sun, Jong Seung Kim

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Abstract

Fluorescence imaging is a non-invasive and highly sensitive bioimaging technique that has shown remarkable strides in plant science. It enables real-time monitoring and analysis of biological and pathological processes in plants by labeling specific molecular or cellular structures with fluorescent probes. However, tissue scattering and phytochrome interference have been obstacles for conventional fluorescence imaging of plants in the ultraviolet and visible spectrum, resulting in unsatisfactory imaging quality. Fortunately, advances in near-infrared (NIR) fluorescence imaging technology (650–900 nm) offer superior spatial-temporal resolution and reduced tissue scattering, which is sure to improve plant imaging quality. In this review, we summarize recent progress in the development of NIR fluorescence imaging probes and their applications for in vivo plant imaging and the identification of plant-related biomolecules. We hope this review provides a new perspective for plant science research and highlights NIR fluorescence imaging as a powerful tool for analyzing plant physiology, adaptive mechanisms, and coping with environmental stress in the near future.

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用近红外荧光探针照亮植物

荧光成像是一种非侵入性、高灵敏度的生物成像技术，在植物科学领域取得了显著进展。它通过用荧光探针标记特定的分子或细胞结构，对植物的生物和病理过程进行实时监测和分析。然而，组织散射和植物色素干扰一直是传统紫外和可见光谱植物荧光成像的障碍，导致成像质量不尽人意。幸运的是，近红外（NIR）荧光成像技术（650-900 nm）的进步提供了卓越的时空分辨率，并减少了组织散射，这必将提高植物成像质量。在本综述中，我们总结了近红外荧光成像探针的最新研发进展及其在植物体内成像和植物相关生物分子鉴定中的应用。我们希望这篇综述能为植物科学研究提供一个新的视角，并强调近红外荧光成像是在不久的将来分析植物生理、适应机制和应对环境胁迫的有力工具。

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来源期刊

Science China Chemistry CHEMISTRY, MULTIDISCIPLINARY-

CiteScore

14.40

自引率

7.30%

发文量

3787

审稿时长

2.2 months

期刊介绍： Science China Chemistry, co-sponsored by the Chinese Academy of Sciences and the National Natural Science Foundation of China and published by Science China Press, publishes high-quality original research in both basic and applied chemistry. Indexed by Science Citation Index, it is a premier academic journal in the field. Categories of articles include: Highlights. Brief summaries and scholarly comments on recent research achievements in any field of chemistry. Perspectives. Concise reports on thelatest chemistry trends of interest to scientists worldwide, including discussions of research breakthroughs and interpretations of important science and funding policies. Reviews. In-depth summaries of representative results and achievements of the past 5–10 years in selected topics based on or closely related to the research expertise of the authors, providing a thorough assessment of the significance, current status, and future research directions of the field.