AKTIVITAS ANTIBAKTERI EKSTRAK TANGKAI Begonia multangula Blume TERHADAP PERTUMBUHAN Aggregatibacter actinomycetemcomitans

Abstract

Periodontitis is the most common dental and oral disease found in society and can cause tooth loss. One of the bacteria that causes periodontitis is Aggregatibacter actinomycetemcomitans. Periodontitis treatment is carried out by mechanical therapy (scaling and root planning) accompanied by chemical therapy (antibiotics and mouthwash). Prolonged use of chemical therapy can cause resistance, impaired taste and discolored teeth. As an alternative, herbal ingredients that have antibacterial properties can be used. Begonia multangula Blume stalks are used in several areas as a medicinal plant and have antibacterial activity. The purpose of this study was to determine the antibacterial activity of Begonia multangula Blume stalk extract on the growth of A. actinomycetemcomitans in vitro. The study was conducted in an experimental laboratory with A. actinomycetemcomitans ATCC 43718 as a sample. Begonia multangula Blume stalks were extracted using the maceration method and 5 concentration series were made (3.12%, 6.25%, 12.5%, 25% and 50%). The antibacterial test was carried out by the paper disc diffusion method with a positive control of 2% Chlorhexidine gluconate and a negative control of 1% DMSO. The diameter of the inhibition zone was then statistically analyzed. The results showed that the antibacterial activity of Begonia multangula Blume stem extract increased with increasing concentration with the highest activity at 50% concentration with an inhibition zone of 20.33 mm (p<0.05). The conclusion of this research is that the ethanol extract of Begonia multangula Blume stalk has antibacterial activity against A. actinomycetemcomitans.