Bio-cementation of tailings sands using ultraviolet induced urease-producing bacteria and its biomineralization mechanism

IF 2.2 4区工程技术 Q3 ENGINEERING, GEOLOGICAL Environmental geotechnics Pub Date : 2023-10-04 DOI:10.1680/jenge.22.00075

Kai Xu, Ming Huang, Jinxuan Zhang, Mingjuan Cui, Chaoshui Xu

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Abstract

The disposal of tailings in a safe and environmentally friendly manner has always been a challenging issue. The microbially induced carbonate precipitation (MICP) technique is used to stabilise tailings sands. MICP is an innovative soil stabilisation technology. However, its field application in tailings sands is limited due to the poor adaptability of non-native urease-producing bacteria (UPB) in different natural environments. In this study, the ultraviolet (UV) mutagenesis technology was used to improve the performance of indigenous UPB, sourced from a hot and humid area of China. Mechanical property tests and microscopic inspections were conducted to assess the feasibility and the effectiveness of the technology. The roles played by the UV-induced UPB in the processes of nucleation and crystal growth were revealed by scanning electron microscopy imaging. The impacts of elements contained in the tailings sands on the morphology of calcium carbonate crystals were studied with Raman spectroscopy and energy-dispersive X-ray spectroscopy. The precipitation pattern of calcium carbonate and the strength enhancement mechanism of bio-cemented tailings were analysed in detail. The stabilisation method of tailings sands described in this paper provides a new cost-effective approach to mitigating the environmental issues and safety risks associated with the storage of tailings.

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紫外光诱导产脲菌的尾矿砂生物胶结及其生物矿化机制

如何以安全、环保的方式处理尾矿一直是一个具有挑战性的问题。采用微生物诱导碳酸盐沉淀(MICP)技术稳定尾矿砂。MICP是一种创新的土壤稳定技术。但由于非天然产脲菌(UPB)对不同自然环境的适应性较差，限制了其在尾矿砂中的应用。本研究利用紫外诱变技术提高了产自中国湿热地区的国产UPB的性能。进行了力学性能测试和显微检查，以评估该技术的可行性和有效性。通过扫描电镜成像揭示了uv诱导的UPB在成核和晶体生长过程中所起的作用。利用拉曼光谱和能量色散x射线光谱研究了尾矿砂中所含元素对碳酸钙晶体形貌的影响。详细分析了碳酸钙的沉淀规律及生物胶结尾砂的强度增强机理。本文描述的尾矿砂稳定方法为减轻尾矿砂储存带来的环境问题和安全风险提供了一种新的经济有效的方法。

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来源期刊

Environmental geotechnics Environmental Science-Water Science and Technology

CiteScore

6.20

自引率

18.20%

发文量

期刊介绍： In 21st century living, engineers and researchers need to deal with growing problems related to climate change, oil and water storage, handling, storage and disposal of toxic and hazardous wastes, remediation of contaminated sites, sustainable development and energy derived from the ground. Environmental Geotechnics aims to disseminate knowledge and provides a fresh perspective regarding the basic concepts, theory, techniques and field applicability of innovative testing and analysis methodologies and engineering practices in geoenvironmental engineering. The journal''s Editor in Chief is a Member of the Committee on Publication Ethics. All relevant papers are carefully considered, vetted by a distinguished team of international experts and rapidly published. Full research papers, short communications and comprehensive review articles are published under the following broad subject categories: geochemistry and geohydrology, soil and rock physics, biological processes in soil, soil-atmosphere interaction, electrical, electromagnetic and thermal characteristics of porous media, waste management, utilization of wastes, multiphase science, landslide wasting, soil and water conservation, sensor development and applications, the impact of climatic changes on geoenvironmental, geothermal/ground-source energy, carbon sequestration, oil and gas extraction techniques, uncertainty, reliability and risk, monitoring and forensic geotechnics.