Botanical validation of Ecclinusa (Chrysophylloideae: Sapotaceae) from forest remnants using molecular analysis as a complementary method to morphological identification
Shirlene Cristina Brito da SILVA, Gleiciane Rodrigues dos SANTOS, Telma Fátima Vieira BATISTA, Gracialda Costa FERREIRA, Alessandra Jackeline Guedes de MORAES, Jéssy Anni Vilhena SENADO, Victor Fernando Galvão BEZERRA, Dênmora Gomes de ARAÚJO, Selma Toyoko OHASHI, Anderson Gonçalves da SILVA
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引用次数: 0
Abstract
ABSTRACT In the context of a forest restoration program in a mining area in the eastern Brazilian Amazon, the objective of this study was to identify the species of trees of abiu-seringarana (Ecclinusa sp.) in forest remnants adjacent to the mine. As the identification of Ecclinusa based on external morphology alone is difficult, it was corroborated by molecular analysis. Leaf samples of three trees were compared with Ecclinusa in the Embrapa herbarium collection. DNA was extracted with a CTAB modified protocol for sequencing with ITS markers. Both the morphological identification and the alignment of the obtained sequences with material in GenBank indicated that the individuals belong to Ecclinusa guianensis, which is confirmed as the species occurring in the mining area.
期刊介绍：
Acta Amzonica is a multidisciplinary, peer-reviewed, open access, free-of-charge scientific journal for research in and about the Amazon region, published since 1971 by the Instituto Nacional de Pesquisas da Amazônia - INPA, in Brazil.
The journal publishes quarterly issues containing articles and short communications in English across a broad range of disciplines, including Agronomy and Forestry, Animal Sciences and Fisheries, Biodiversity and Conservation, Biotechnology, Chemistry and Pharmacology, Environmental Sciences, Food Sciences, Geosciences, Health Sciences, Human and Social Sciences, and Materials Technology.
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