Antitumor Activity of Ziziphus Jujube Fruit Extracts in KG-1 and NALM-6 Acute Leukemia Cell Lines

Shiva Mosadegh Manshadi, Mohammad Reza Shams Ardekani
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 Materials and Methods: In this experimental study, the aqueous, ethyl acetate, and hydroalcoholic extracts of the Ziziphus jujube were prepared. Total phenolic and flavonoid components were detected because the presence of these compounds is associated with antioxidant and anticancer effects. Different concentrations of extracts were prepared, and KG-1 and NALM-6 cell lines were treated with them at 12, 24, 36, and 48 hours. Cell viability and IC50 values of the extracts were calculated using MTT assays. BD Cycle TEST PLUS DNA Kit was used for cell cycle progression analysis. Bcl2, Bax, and caspase-3 mRNA expressions were also assessed.
 Results: Cell viability decreased in a concentration-dependent manner. The best efficacy belonged to the ethyl acetate extract. Investigation of cell cycle progression demonstrated that the number of G0/G1 cells enhanced and the number of G2/M cells decreased when the ethyl acetate extract was applied in its IC50 concentration. A considerable increase in Caspase-3 and Bax and a decrease in Bcl2 gene expression were detected in molecular examination.
 Conclusion: According to our research, Ziziphus jujube ethyl acetate extract has antitumor properties on KG-1 and NALM-6 cell lines, possibly through induction of apoptosis and cell cycle regulation.","PeriodicalId":94048,"journal":{"name":"International journal of hematology-oncology and stem cell research","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of hematology-oncology and stem cell research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18502/ijhoscr.v17i4.13917","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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Abstract

Background: Ziziphus jujube Mill. belongs to the Rhamnaceae family. It has been reported to have a variety of biological activities such as antitumor, antioxidant, and anti-inflammatory effects. This study investigates the antiproliferative effect of Ziziphus jujube on KG-1 and NALM-6 acute leukemia cell lines. Materials and Methods: In this experimental study, the aqueous, ethyl acetate, and hydroalcoholic extracts of the Ziziphus jujube were prepared. Total phenolic and flavonoid components were detected because the presence of these compounds is associated with antioxidant and anticancer effects. Different concentrations of extracts were prepared, and KG-1 and NALM-6 cell lines were treated with them at 12, 24, 36, and 48 hours. Cell viability and IC50 values of the extracts were calculated using MTT assays. BD Cycle TEST PLUS DNA Kit was used for cell cycle progression analysis. Bcl2, Bax, and caspase-3 mRNA expressions were also assessed. Results: Cell viability decreased in a concentration-dependent manner. The best efficacy belonged to the ethyl acetate extract. Investigation of cell cycle progression demonstrated that the number of G0/G1 cells enhanced and the number of G2/M cells decreased when the ethyl acetate extract was applied in its IC50 concentration. A considerable increase in Caspase-3 and Bax and a decrease in Bcl2 gene expression were detected in molecular examination. Conclusion: According to our research, Ziziphus jujube ethyl acetate extract has antitumor properties on KG-1 and NALM-6 cell lines, possibly through induction of apoptosis and cell cycle regulation.
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红枣果实提取物对KG-1和NALM-6急性白血病细胞的抗肿瘤活性
背景:Ziziphus枣厂。属于鼠李科。据报道,它具有多种生物活性,如抗肿瘤、抗氧化和抗炎作用。本研究探讨了红枣对急性白血病细胞KG-1和NALM-6的抑制作用。材料与方法:以红枣为原料,分别制备水提物、乙酸乙酯和水醇提物。检测总酚和类黄酮成分,因为这些化合物的存在与抗氧化和抗癌作用有关。制备不同浓度的提取物,分别于12、24、36、48 h作用于KG-1和NALM-6细胞株。采用MTT法计算提取液的细胞活力和IC50值。采用BD Cycle TEST PLUS DNA Kit进行细胞周期进程分析。同时检测Bcl2、Bax和caspase-3 mRNA的表达。 结果:细胞活力呈浓度依赖性下降。以乙酸乙酯提取物效果最佳。细胞周期进程的研究表明,当乙酸乙酯提取物在IC50浓度下作用时,G0/G1细胞数量增加,G2/M细胞数量减少。分子检查发现Caspase-3和Bax基因表达明显升高,Bcl2基因表达明显降低。 结论:酸枣乙酸乙酯提取物对KG-1和NALM-6细胞具有抗肿瘤作用,可能是通过诱导细胞凋亡和调节细胞周期来实现的。
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