Monitoring of the Enzymatic Reactions Course by Differential Microwave Dielectrometry Method in Real Time

IF 0.6 Q4 PHYSICS, MULTIDISCIPLINARY Ukrainian Journal of Physics Pub Date : 2023-10-20 DOI:10.15407/ujpe68.9.608
K.S. Kuznetsova, V.A. Pashynska, Z.E. Eremenko, O.I. Shubnyi, A.V. Martynov, A.A. Prokopenko
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Abstract

Enzymatic reactions are the basis of many biotechnological manufacturing and biomedical diagnostic procedures that require effective methods of monitoring over the reaction course. In the current paper, we present the results of the development of a new approach within the differential microwave dielectrometry method for the non-invasive monitoring of the course of enzymatic reactions based on the complex permittivity changes of these reactive mixture solutions in real time at a fixed frequency of 31.82 GHz. The dynamic studies of the dielectric parameters of selected enzymatic systems containing a protein substrate (immunoglobulin G, human serum albumin) and enzyme trypsin. The developed differential microwave dielectrometry setup has been performed to verify the proposed approach effectiveness for the enzymatic reaction monitoring in biomedical practice and the food industry. Our microwave dielectrometry results have been validated by the results of the UV-Vis spectrophotometry method for selected enzymatic systems. We propose a new approach to use the differential microwave dielectrometry method with high sensitivity (in average 0.5% and 3÷5% for the real and imaginary parts of the complex permittivity, respectively) to estimate the course of enzymatic reactions in real time.
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差分微波介电法实时监测酶促反应过程
酶促反应是许多生物技术制造和生物医学诊断程序的基础,需要对反应过程进行有效监测的方法。在本文中,我们提出了一种基于这些反应混合溶液在31.82 GHz固定频率下的复介电常数变化的实时无创监测酶促反应过程的差分微波介电法新方法的发展结果。含有蛋白质底物(免疫球蛋白G,人血清白蛋白)和胰蛋白酶的选定酶系统的介电参数的动态研究。开发的差分微波介电测量装置已被执行,以验证所提出的方法在生物医学实践和食品工业中酶促反应监测的有效性。我们的微波介电测量结果已通过紫外可见分光光度法对所选酶体系的结果进行了验证。我们提出了一种新的方法,利用高灵敏度的差分微波介电法(复介电常数的实部和虚部平均分别为0.5%和3÷5%)实时估计酶促反应的过程。
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来源期刊
Ukrainian Journal of Physics
Ukrainian Journal of Physics PHYSICS, MULTIDISCIPLINARY-
CiteScore
1.20
自引率
20.00%
发文量
244
期刊介绍: Ukrainian Journal of Physics is the general physics edition of the Department of Physics and Astronomy of the National Academy of Sciences of Ukraine. The journal publishes original papers and reviews in the fields of experimental and theoretical physics.
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