A Comparative In Vitro Study: Cytotoxicity Effect of Curcumin Vs Biodegradable Nanoparticles/PVA Against HNO97 And Effect of Curcumin on Cell Cycle Phases

Abstract

Purpose: This study evaluated that curcumin and biodegradable nanoparticles with PVA as capping material, in terms of cytotoxicity followed by cell cycle analysis against the HNO97 cell line (tongue carcinoma). Material and methods: 5mg of pure curcumin, 7.5 mg of Nano curcumin formulation, and SRB for IC50 done on five concentrations. A flow cytometry test was carried on to evaluate the effect of curcumin on the HNO97 cell line. Results: This study postulated that curcumin had the highest cytotoxicity effect against cell line (HNO97) with IC50=1.59 µg/ml in comparison to curcumin nanoparticles/PVA showed low cytotoxicity owing to the pH (7.4) and surface charges of PVA. Also, the cell cycle test for curcumin showed that the percentage of cells in G2/M phase was significantly increased (p=0.003). Conclusion: The study showed that curcumin has a powerful anticancer effect against in vitro tongue carcinoma. Generally, these results reported that curcumin can in vitro inhibit cancer cells.