{"title":"Preparation of carrier free [125I]IodoHoechst 33258","authors":"Roger F. Martin, Marshall Pardee","doi":"10.1016/0020-708X(85)90049-3","DOIUrl":null,"url":null,"abstract":"<div><p>The DNA ligand Hoechst 33258 has been iodinated with carrier-free [<sup>125</sup>I]iodide by the lactoperoxidase method. The product was separated from other iodination products and the uniodinated compound by preparative thin layer chromatography. <sup>125</sup>I-labelled Hoechst 33258 is useful as a probe with DNA molecules of defined sequence since the decay of the <sup>125</sup>I atom results in the induction of a double-strand break, the location of which can be detected by DNA-sequencing techniques.</p></div>","PeriodicalId":22517,"journal":{"name":"The International journal of applied radiation and isotopes","volume":"36 9","pages":"Pages 745-747"},"PeriodicalIF":0.0000,"publicationDate":"1985-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-708X(85)90049-3","citationCount":"15","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The International journal of applied radiation and isotopes","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0020708X85900493","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 15
Abstract
The DNA ligand Hoechst 33258 has been iodinated with carrier-free [125I]iodide by the lactoperoxidase method. The product was separated from other iodination products and the uniodinated compound by preparative thin layer chromatography. 125I-labelled Hoechst 33258 is useful as a probe with DNA molecules of defined sequence since the decay of the 125I atom results in the induction of a double-strand break, the location of which can be detected by DNA-sequencing techniques.
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