Review of antimicrobial spectrum of Tridax procumbens L. (Vishalyakarani) and estimation of flavonoids & phenols present in its leaves in ninety six well plate methods

Shiva Prasad Mohanthy, Kshirabdhi Tanaya Rautaray, Dr. Raka Jain
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Abstract

Vishalyakarani or Tridax procumbens L is an Ayurvedic herb under asteraceae family with traditional use in wound healing purpose. It has many pharmacological properties including anti-microbial, anti-cancer, wound healing and anti-septic. The present study is a combined study consisting reviewing the anti-microbial spectrum of Tridax procumbens L. (Vishalyakarani) and quantification of flavonoids and phenols from its leaves especially from the Gandhamardhan hill, Odisha. This aims to equip researchers a useful data about the chemical contents of T. procumbens. The extract was prepared by using Soxhlet extractor and concentrated by distillation. Phytochemical analysis revealed the presence of flavonoids, phenols in extract and quantification by the high-throughput nine sixty well plate method. The review process yielded 748 research papers and after thorough discussion 81 papers were selected. The plant has many chemical compounds including flavonoids, essential oils, phenols and wide range of pharmacological activities from which we have included the anti-microbial activities. Historically and from Ramayana we have heard about the effectiveness of the Vishalyakarani in wound healing and in present review proves its worth. But there was a lack of quantification of two important phyto contents and it’s done by high-throughput 96-well plate method.
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原甘油三酯抗菌谱研究进展及黄酮类化合物含量评价96种孔板法测定其叶片中的酚类物质
Vishalyakarani或triidax procumbens L是一种阿育吠陀草药,属于菊科,传统用于伤口愈合。它具有抗微生物、抗癌、伤口愈合和抗菌等药理特性。本研究是对产自印度奥里萨邦Gandhamardhan山的原甘豆(triidax procumbens L., Vishalyakarani)的抗微生物光谱进行综述,并对其叶片中黄酮类和酚类物质进行定量分析。这样做的目的是为研究人员提供有关原藜的化学成分的有用数据。提取液采用索氏提取器提取,蒸馏浓缩。植物化学分析发现提取物中含有黄酮类和酚类化合物,并采用高通量九六孔板法进行定量分析。评审过程共产生了748篇研究论文,经过深入讨论,最终选定了81篇。该植物具有多种化学成分,包括黄酮类化合物、精油、酚类化合物和广泛的药理活性,其中包括抗微生物活性。从历史上和从罗摩衍那中,我们听说了维沙利亚卡拉尼在伤口愈合方面的有效性,并在目前的审查中证明了它的价值。但缺少两种重要植物含量的定量,采用高通量96孔板法。
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