Mercedes P. Silva, Daniel H. Basigalup, Stella Maris Chiacchiera, Verónica Trucco, Fabián Giolitti, Eva Mamani, Carolina del Pilar Díaz, Milena E. Manzur
{"title":"Effects of infection with the viral complex alfalfa dwarf disease on productivity traits and coumestrol production in alfalfa plants","authors":"Mercedes P. Silva, Daniel H. Basigalup, Stella Maris Chiacchiera, Verónica Trucco, Fabián Giolitti, Eva Mamani, Carolina del Pilar Díaz, Milena E. Manzur","doi":"10.1071/cp23143","DOIUrl":null,"url":null,"abstract":"Context Viral infections generate yield losses in alfalfa due to the impact on growth, seed production, impaired nitrogen fixation and fungal disease susceptibility, but also stimulate production of coumestrol, which can exhibit oestrogenic activity in livestock and human.Aims We investigated the change in coumestrol content and its relationship with morphological and productivity traits in alfalfa plants infected with alfalfa viral complex.Methods Seven genotypes from cultivar Monarca SP-INTA and six of Traful PV-INTA were used. Plants were cloned and treated as non-infected and infected with alfalfa viral complex (alfalfa dwarf disease). Productivity and morphological traits were assessed. Disease symptoms were used to estimate the genotype severity index (GSI, %), coumestrol content was determined by high pressure liquid chromatography.Key results Viral infection reduced internode distance (iD) for the majority of Monarca genotypes, whereas in Traful one was affected, generating dwarfism. The iD reduction positively correlated with GSI only in Monarca genotypes. Coumestrol content varied between genotypes of each of the two cultivars. Monarca genotypes ranged between 350 and 1140 (infected and non-infected) ppm, and Traful genotypes ranged between 0 and 942 (infected and non-infected) ppm. However, viral infection promoted coumestrol increase in three genotypes only.Conclusions All Monarca genotypes studied seem to be more sensitive to viral infections than those of Traful, as GSI demonstrated. Independently of cultivar, coumestrol content increased for three genotypes. High coumestrol content was correlated with low GSI.Implications The knowledge of genotype response to viral infections would be useful for breeders to select superior genotypes as well as to originate new cultivars either as forage or as natural sources of phytoestrogens.","PeriodicalId":51237,"journal":{"name":"Crop & Pasture Science","volume":null,"pages":null},"PeriodicalIF":1.8000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Crop & Pasture Science","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1071/cp23143","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Context Viral infections generate yield losses in alfalfa due to the impact on growth, seed production, impaired nitrogen fixation and fungal disease susceptibility, but also stimulate production of coumestrol, which can exhibit oestrogenic activity in livestock and human.Aims We investigated the change in coumestrol content and its relationship with morphological and productivity traits in alfalfa plants infected with alfalfa viral complex.Methods Seven genotypes from cultivar Monarca SP-INTA and six of Traful PV-INTA were used. Plants were cloned and treated as non-infected and infected with alfalfa viral complex (alfalfa dwarf disease). Productivity and morphological traits were assessed. Disease symptoms were used to estimate the genotype severity index (GSI, %), coumestrol content was determined by high pressure liquid chromatography.Key results Viral infection reduced internode distance (iD) for the majority of Monarca genotypes, whereas in Traful one was affected, generating dwarfism. The iD reduction positively correlated with GSI only in Monarca genotypes. Coumestrol content varied between genotypes of each of the two cultivars. Monarca genotypes ranged between 350 and 1140 (infected and non-infected) ppm, and Traful genotypes ranged between 0 and 942 (infected and non-infected) ppm. However, viral infection promoted coumestrol increase in three genotypes only.Conclusions All Monarca genotypes studied seem to be more sensitive to viral infections than those of Traful, as GSI demonstrated. Independently of cultivar, coumestrol content increased for three genotypes. High coumestrol content was correlated with low GSI.Implications The knowledge of genotype response to viral infections would be useful for breeders to select superior genotypes as well as to originate new cultivars either as forage or as natural sources of phytoestrogens.
期刊介绍:
Crop and Pasture Science (formerly known as Australian Journal of Agricultural Research) is an international journal publishing outcomes of strategic research in crop and pasture sciences and the sustainability of farming systems. The primary focus is broad-scale cereals, grain legumes, oilseeds and pastures. Articles are encouraged that advance understanding in plant-based agricultural systems through the use of well-defined and original aims designed to test a hypothesis, innovative and rigorous experimental design, and strong interpretation. The journal embraces experimental approaches from molecular level to whole systems, and the research must present novel findings and progress the science of agriculture.
Crop and Pasture Science is read by agricultural scientists and plant biologists, industry, administrators, policy-makers, and others with an interest in the challenges and opportunities facing world agricultural production.
Crop and Pasture Science is published with the endorsement of the Commonwealth Scientific and Industrial Research Organisation (CSIRO) and the Australian Academy of Science.