Application of Wox2a in transformation of recalcitrant maize genotypes

IF 4.6 4区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY aBIOTECH Pub Date : 2023-10-13 DOI:10.1007/s42994-023-00116-6
Qiangbo Liu, Xian Sheng Zhang, Ying Hua Su
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Abstract

The genetic transformation plays an important role in plant gene functional analysis and its genetic improvement. However, only a limited number of maize germplasms can be routinely transformed. The maize gene Wuschel-like homeobox protein 2a (Wox2a) was shown to play a crucial role in promoting the formation of embryonic cells and enhancing the efficiency of genetic transformation in maize. This commentary discusses the mechanism by which the Wox2a gene contributes to the variation in embryogenic tissue culture response among different maize inbred lines. In addition, the frequency and intensity of Wox2a or Wus2/Bbm vector-induced somatic embryogenesis was also discussed. The application of Wox2a in transformation of recalcitrant maize genotypes could well accelerate the development of maize genetic improvement.

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将 Wox2a 应用于转化难以克服的玉米基因型
基因转化在植物基因功能分析和遗传改良中发挥着重要作用。然而,只有数量有限的玉米种质可以进行常规转化。研究表明,玉米基因 Wuschel-like homeobox protein 2a (Wox2a) 在促进玉米胚胎细胞的形成和提高遗传转化效率方面发挥着重要作用。本评论探讨了 Wox2a 基因导致不同玉米近交系胚胎组织培养反应差异的机制。此外,还讨论了 Wox2a 或 Wus2/Bbm 载体诱导体细胞胚胎发生的频率和强度。将 Wox2a 应用于转化顽固的玉米基因型可加速玉米遗传改良的发展。
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来源期刊
CiteScore
7.70
自引率
2.80%
发文量
0
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