{"title":"Stability Indicating Assay Method Development and Validation of Sitagliptin and Metformin Combination Tablets by Reverse Phase - HPLC","authors":"Gogada V.P Rao, Meka Lingam, Bavisetti Lakshmi, Anantha L Vadivelu, Duvvuri Suryakala","doi":"10.25258/ijpqa.14.3.19","DOIUrl":null,"url":null,"abstract":"Two pharmaceutical compounds were isolated, separated, and confirmed using chromatographic techniques. The method involved utilizing an inertsil phenyl analytical the analytical column has dimensions of 250 mm x 4.0 mm and contains particles with a size of 5 μm. A solution is filled as a buffer, combined with a mobile phase containing a grid. with a pH of 4.0 made from potassium dihydrogen phosphate, orthophosphoric acid and of acetonitrile in a 70:30 ratio 0.8 mL per minute. The column temperature is maintained at 30℃ as part of the setup. The detection of the two drugs is continuously monitored using this setup. A wavelength of 210 nm using a UV detector with injection volume 20 μL. Forced degradation studies are carried out using PDA detector. Peak homogeneity explained as peak purity using lab solution software","PeriodicalId":14260,"journal":{"name":"International Journal of Pharmaceutical Quality Assurance","volume":"5 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Pharmaceutical Quality Assurance","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.25258/ijpqa.14.3.19","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Pharmacology, Toxicology and Pharmaceutics","Score":null,"Total":0}
引用次数: 0
Abstract
Two pharmaceutical compounds were isolated, separated, and confirmed using chromatographic techniques. The method involved utilizing an inertsil phenyl analytical the analytical column has dimensions of 250 mm x 4.0 mm and contains particles with a size of 5 μm. A solution is filled as a buffer, combined with a mobile phase containing a grid. with a pH of 4.0 made from potassium dihydrogen phosphate, orthophosphoric acid and of acetonitrile in a 70:30 ratio 0.8 mL per minute. The column temperature is maintained at 30℃ as part of the setup. The detection of the two drugs is continuously monitored using this setup. A wavelength of 210 nm using a UV detector with injection volume 20 μL. Forced degradation studies are carried out using PDA detector. Peak homogeneity explained as peak purity using lab solution software
用色谱技术分离、分离和确认了两种药物化合物。该方法使用惯性苯基分析柱，分析柱尺寸为250 mm x 4.0 mm，包含尺寸为5 μm的颗粒。溶液被填充为缓冲器，与包含网格的移动相结合。pH为4.0，由磷酸二氢钾、正磷酸和乙腈按70:30的比例配制，每分钟0.8 mL。作为设置的一部分，色谱柱温度保持在30℃。使用该装置连续监测两种药物的检测。波长为210 nm，进样量为20 μL。使用PDA检测器进行强制降解研究。使用实验室溶液软件将峰均匀性解释为峰纯度
期刊介绍：
INTERNATIONAL JOURNAL OF PHARMACEUTICAL QUALITY ASSURANCE is a quarterly international journal publishing the finest peer-reviewed research in the field of Pharmaceutical Quality Assurance and Pharmaceutical Analysis on the basis of its originality, importance, disciplinary interest, timeliness, accessibility, elegance, and surprising conclusions. IJPQA also provides rapid, authoritative, insightful and arresting news and interpretation of topical and coming trends affecting science, scientists and the wider public.
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