Saima Khan, Sharada Mallubhotla, Malik Muzafar Manzoor, Sandeep Singh Pagoch, Ashok Ahuja
{"title":"Dynamics of acid phosphatase production by cell suspension system and its further characterization","authors":"Saima Khan, Sharada Mallubhotla, Malik Muzafar Manzoor, Sandeep Singh Pagoch, Ashok Ahuja","doi":"10.9755/ejfa.2023.3141","DOIUrl":null,"url":null,"abstract":"The current study compares the influence of media engineered media on production of acid phophatase (APase) with its characterization in suspension cell cultures of Atropa acuminata, authenticated by rbcL and ITS. Various parameters like pH, inoculum size, temperature, nutrient sources in the medium were optimized for APase hyperproduction in in vitro conditions. APase production was accomplished to be evidently amplified at 5% inoculum size (94.90 U/gFW), pH 5.0(99.75 U/gFW) and incubation temperature of 30°C (91.5U/gFW). Sucrose as carbon and ammonium nitrate as nitrogen sources were eminently suited for maximum enzyme harvest (91.20 U/gFW and 94.56 U/gFW). The APase activity was observed to be distinctly increased in phosphate (Pi) starved suspension cells (197.84 U/gFW). Overall, 6.5 fold increase in APase activity was achieved as compared to the activity before optimization studies. Hence the present Apase production and its activity from Atropa acuminata suspension culture may have apparent use for future industrial, agricultural and biotechnological application. Keywords: Acuminata; Acid phosphatase; p-nitrophenylphosphate; Suspension cells; Pi-starvation","PeriodicalId":11648,"journal":{"name":"Emirates Journal of Food and Agriculture","volume":null,"pages":null},"PeriodicalIF":0.7000,"publicationDate":"2023-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Emirates Journal of Food and Agriculture","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.9755/ejfa.2023.3141","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 0
Abstract
The current study compares the influence of media engineered media on production of acid phophatase (APase) with its characterization in suspension cell cultures of Atropa acuminata, authenticated by rbcL and ITS. Various parameters like pH, inoculum size, temperature, nutrient sources in the medium were optimized for APase hyperproduction in in vitro conditions. APase production was accomplished to be evidently amplified at 5% inoculum size (94.90 U/gFW), pH 5.0(99.75 U/gFW) and incubation temperature of 30°C (91.5U/gFW). Sucrose as carbon and ammonium nitrate as nitrogen sources were eminently suited for maximum enzyme harvest (91.20 U/gFW and 94.56 U/gFW). The APase activity was observed to be distinctly increased in phosphate (Pi) starved suspension cells (197.84 U/gFW). Overall, 6.5 fold increase in APase activity was achieved as compared to the activity before optimization studies. Hence the present Apase production and its activity from Atropa acuminata suspension culture may have apparent use for future industrial, agricultural and biotechnological application. Keywords: Acuminata; Acid phosphatase; p-nitrophenylphosphate; Suspension cells; Pi-starvation
期刊介绍:
The "Emirates Journal of Food and Agriculture [EJFA]" is a unique, peer-reviewed Journal of Food and Agriculture publishing basic and applied research articles in the field of agricultural and food sciences by the College of Food and Agriculture, United Arab Emirates University, United Arab Emirates.