Isolation and Characterization of Rhizospheric Soil Bacteria Present in Shivrajpur Mn Mine Site

Abstract

AbstractDue to the obvious potential hazards it poses to the environment, animals, and human health, Mn (II) contamination has become worrisome. The broad aim of the study was to examine the contamination levels of soil in the Shivrajpur Mn mine site, physicochemical tests such as pH and heavy metal analyses were done. Later 16S rRNA sequencing was used to characterize bacterial species isolated from different colonizer plant samples. The bacterial strains recovered from Mn mine rhizospheric soil exemplified morphological and molecular diversity. It was discovered that all seven bacterial isolates could survive Mn salt concentrations up to 2000 mg/l, whereas only one, Staphylococcus capitis (GenBank OP861008), could withstand concentrations up to 10,000 mg/l. The three isolates, Staphylococcus capitis, Bacillus albus (GenBank OP861099), Bacillus tequilensis (GenBank OQ135205), have the ability to sustain the Mn tolerance as the agar wells were surrounded by growth expanse. The findings imply that the Staphylococcus capitis could be used to create a biosorbent that is both effective and environmentally benign, and it can aid in the bioremediation of contaminated soil.Keywords: BacteriabioremediationMn contaminationmolecular diversity16SrRNA sequencing AcknowledgementsWe are grateful to Prof. Kauresh Vachhrajani, Former Head and Professor of Zoology, The Maharaja Sayajirao University of Baroda for his vision and guiding force for taking up this initiative. We also express our gratitude to Prof. P. Padmaja Sudhakar, Officiating Head, Department of Environmental Studies and Prof. H.R. Kataria, Dean, Faculty of Science, The Maharaja Sayajirao University of Baroda for constant support and encouragement.Disclosure statementNo potential conflict of interest was reported by the author(s).Additional informationFundingThis work was supported by Research and Consultancy Cell, The Maharaja Sayajirao University of Baroda.