High throughput sequencing data reveals the complete mitogenome, abundance, maternal phylogeny, and mitogenomic diversity of Alectoris chukar of Iraq

IF 0.7 4区 农林科学 Q3 AGRONOMY Emirates Journal of Food and Agriculture Pub Date : 2023-08-31 DOI:10.9755/ejfa.2023.3143
Paiman Yousif, Hevidar Taha
{"title":"High throughput sequencing data reveals the complete mitogenome, abundance, maternal phylogeny, and mitogenomic diversity of Alectoris chukar of Iraq","authors":"Paiman Yousif, Hevidar Taha","doi":"10.9755/ejfa.2023.3143","DOIUrl":null,"url":null,"abstract":"Alectoris chukar is a wild game bird found in the north of Iraq, near the center of domestication and diversity of species. The mitogenome is one of the most vital resources for comprehensive studies of genetic diversity and molecular evolutionary relationships among avian species. In this study, we used whole genome sequencing raw reads and bioinformatics analysis to sequence and assemble Alectoris chukar's complete mitogenome for the first time. We also studied the maternal lineage and phylogenetic position of Alectoris chukar, as well as some mitogenomic diversity parameters. As a result, the complete mitogenomes with a length ranging from 16686 bp to 16688 bp of four individuals of wild Alectoris chukar were sequenced and assembled. They have a typical avian mitogenome structure with 2 ribosomal RNA (rRNA), 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, and one non-coding control region. Our findings from bioinformatics analysis remarkably demonstrated that copies of the mitogenome are more abundant in liver tissues than in blood and in the liver tissues of females than in males. The results of phylogenetic analysis clustered the studied mitogenomes with Alectoris chukar as a monophyletic clade. Moreover, in comparison to the different genera, Alectoris chukar showed a high level of mitogenomic similarities to the snowcock species of the genus Tetraogallus within the Phasianidae family. However, they were more distant from other partridges. Additionally, a high percentage of mitogenomic pairwise identities within Iraqi Alectoris chukar and high mitogenomic variations compared to Chinese populations were discovered. The number and location of polymorphic sites indicated that the majority of the mitogenome sequences were conserved, with the control region, ND5, and CYTB genes having the most polymorphic sites. Analyses of phylogenetic and mitogenomic diversity revealed that samples of Alectoris chukar from Iraq have a unique maternal lineage and mitogenomic diversity specific to their geographic distribution, suggesting an Alectoris chukar kurdestanica subspecies. The molecular findings presented here provide valuable knowledge and mitogenomic resources into the evolutionary relationships of Alectoris chukar from the Middle East to avian species in the Phasianidae family.
 Key words: DNA sequencing; Mitochondrial genome; Partridges; Phasianidae; Phylogeny
","PeriodicalId":11648,"journal":{"name":"Emirates Journal of Food and Agriculture","volume":null,"pages":null},"PeriodicalIF":0.7000,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Emirates Journal of Food and Agriculture","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.9755/ejfa.2023.3143","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 0

Abstract

Alectoris chukar is a wild game bird found in the north of Iraq, near the center of domestication and diversity of species. The mitogenome is one of the most vital resources for comprehensive studies of genetic diversity and molecular evolutionary relationships among avian species. In this study, we used whole genome sequencing raw reads and bioinformatics analysis to sequence and assemble Alectoris chukar's complete mitogenome for the first time. We also studied the maternal lineage and phylogenetic position of Alectoris chukar, as well as some mitogenomic diversity parameters. As a result, the complete mitogenomes with a length ranging from 16686 bp to 16688 bp of four individuals of wild Alectoris chukar were sequenced and assembled. They have a typical avian mitogenome structure with 2 ribosomal RNA (rRNA), 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, and one non-coding control region. Our findings from bioinformatics analysis remarkably demonstrated that copies of the mitogenome are more abundant in liver tissues than in blood and in the liver tissues of females than in males. The results of phylogenetic analysis clustered the studied mitogenomes with Alectoris chukar as a monophyletic clade. Moreover, in comparison to the different genera, Alectoris chukar showed a high level of mitogenomic similarities to the snowcock species of the genus Tetraogallus within the Phasianidae family. However, they were more distant from other partridges. Additionally, a high percentage of mitogenomic pairwise identities within Iraqi Alectoris chukar and high mitogenomic variations compared to Chinese populations were discovered. The number and location of polymorphic sites indicated that the majority of the mitogenome sequences were conserved, with the control region, ND5, and CYTB genes having the most polymorphic sites. Analyses of phylogenetic and mitogenomic diversity revealed that samples of Alectoris chukar from Iraq have a unique maternal lineage and mitogenomic diversity specific to their geographic distribution, suggesting an Alectoris chukar kurdestanica subspecies. The molecular findings presented here provide valuable knowledge and mitogenomic resources into the evolutionary relationships of Alectoris chukar from the Middle East to avian species in the Phasianidae family. Key words: DNA sequencing; Mitochondrial genome; Partridges; Phasianidae; Phylogeny
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
高通量测序数据揭示了伊拉克Alectoris chukar的完整有丝分裂基因组,丰度,母系系统发育和有丝分裂基因组多样性
Alectoris chukar是一种野生猎鸟,发现于伊拉克北部,靠近物种驯化和多样性中心。有丝分裂基因组是全面研究鸟类遗传多样性和分子进化关系的重要资源之一。本研究首次采用全基因组测序和生物信息学分析的方法,对楚卡阿立托里斯有丝分裂全基因组进行了测序和组装。我们还研究了楚卡喙鸟的母系和系统发育位置,以及一些有丝分裂基因组多样性参数。结果,对4个野生楚卡阿利托利亚个体的全长为16686 ~ 16688 bp的全有丝分裂基因组进行了测序和组装。它们具有典型的鸟类有丝分裂基因组结构,包括2个核糖体RNA (rRNA)、13个蛋白质编码基因(PCGs)、22个转移RNA (tRNA)基因和一个非编码控制区。我们从生物信息学分析中发现,有丝分裂基因组的拷贝在肝脏组织中比在血液中更丰富,在女性的肝脏组织中比在男性的肝脏组织中更丰富。系统发育分析的结果表明,所研究的有丝分裂基因组与楚卡角翅虫属于单系进化分支。此外,在不同属的比较中,Alectoris chukar显示出高度的丝分裂基因组相似性。然而,它们与其他鹧鸪的距离更远。此外,与中国人群相比,伊拉克阿勒克托里斯chukar人群中有丝分裂基因组成对认同的比例很高,有丝分裂基因组变异也很高。多态性位点的数量和位置表明,大多数有丝分裂基因组序列是保守的,其中控制区、ND5和CYTB基因具有最多的多态性位点。系统发育和有丝分裂基因组多样性分析表明,伊拉克的阿勒克托里斯chukar样品具有独特的母系血统和地理分布特有的有丝分裂基因组多样性,表明其为阿勒克托里斯chukar kurdestanica亚种。本文的分子研究结果为研究中东鸦爪鸟与雉科鸟类的进化关系提供了宝贵的知识和有丝分裂基因组学资源。关键词:DNA测序;线粒体基因组;鹧鸪;雉科;发展史# x0D;
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Emirates Journal of Food and Agriculture
Emirates Journal of Food and Agriculture AGRONOMYFOOD SCIENCE & TECHNOLOGY&nb-FOOD SCIENCE & TECHNOLOGY
CiteScore
1.80
自引率
0.00%
发文量
18
期刊介绍: The "Emirates Journal of Food and Agriculture [EJFA]" is a unique, peer-reviewed Journal of Food and Agriculture publishing basic and applied research articles in the field of agricultural and food sciences by the College of Food and Agriculture, United Arab Emirates University, United Arab Emirates.
期刊最新文献
Exploring the synergistic toxicity of synthetic pesticides and their impact on development and behavior of Honeybee (Apis mellifera L.) Corn and cassava flours can replace wheat flour in gluten-free fish fingers Microbiological quality of raw milk under a scheme of good milking and storage practices in a goat production system in the Lagunera Region, Mexico Effects of lignite-based sulphur fertilizer levels on soil properties and growth of Brassica napus Global warming’s grip on agriculture: Strategies for sustainable production amidst climate change using regression based prediction
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1