Chronic in vitro thermal stress alters functional response of peripheral blood mononuclear cells of Murrah buffalo

IF 1 4区生物学 Q3 BIOLOGY Biological Rhythm Research Pub Date : 2023-10-17 DOI:10.1080/09291016.2023.2267949

Thangaraju Kavin, Venkatasubramanian Leela, Gopalakrishnan Suganya, Senthilkumar TMA, Kandasamy Rajamanickam

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Abstract

ABSTRACT The current study aims to describe the functional changes in Murrah buffalo PBMCs during chronic in-vitro thermal stress. Thus, PBMCs were stimulated at 34°C or 37°C or 40°C for 0 hr, 24 hrs and 48 hrs. At the end of each treatment, mRNA abundance of genes related to heat shock (HSP10, HSP40, HSP60, HSP70, HSP90 and HSF1), inflammation (IL1β, IL6, IL 12 and INF γ), cell viability (Bax and Bcl2) and natural resistance-associated macrophage protein (SLC11A1) were quantified by qPCR. The results revealed upregulation (p < 0.05) in mRNA expression of HSP10, HSP40, HSP60, HSP70, HSF1, IL1β, IL6, IL12, INF γ, BAX, BCL2 and SLC11A1 gene in chronically (24 and 48 hrs) thermal stressed (40 °C) PBMCs than cells maintained at 34 °C or 37 °C. In conclusion, chronic thermal stress directly affects the functionality of Murrah buffalo PBMCs and is likely responsible for immune alterations during thermal stress.KEYWORDS: Heat stressimmune cellsgene expressioninflammationcell viability Disclosure statementNo potential conflict of interest was reported by the author(s).Additional informationFundingThe work was supported by the Tamilnadu Veterinary and Animal Sciences University.

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慢性体外热应激改变水牛外周血单核细胞的功能反应

本研究旨在描述默拉水牛pbmc在慢性体外热应激下的功能变化。因此，分别在34°C、37°C或40°C下刺激pbmc 0小时、24小时和48小时。在每次处理结束时，通过qPCR定量热休克相关基因(HSP10、HSP40、HSP60、HSP70、HSP90和HSF1)、炎症(IL - 1β、IL - 6、IL - 12和INF - γ)、细胞活力(Bax和Bcl2)和天然耐药相关巨噬细胞蛋白(SLC11A1)的mRNA丰度。结果显示，长期(24和48 h)热应激(40℃)PBMCs中HSP10、HSP40、HSP60、HSP70、HSF1、IL1β、IL6、IL12、INF γ、BAX、BCL2和SLC11A1基因的mRNA表达比34℃和37℃时上调(p < 0.05)。综上所述，慢性热应激直接影响默拉水牛pbmc的功能，并可能导致热应激期间的免疫改变。关键词:热应激免疫细胞基因表达炎症细胞活力披露声明作者未报告潜在的利益冲突。这项工作得到了泰米尔纳德邦兽医和动物科学大学的支持。

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来源期刊

Biological Rhythm Research 生物-生理学

CiteScore

3.00

自引率

9.10%

发文量

审稿时长

6-12 weeks

期刊介绍： The principal aim of Biological Rhythm Research is to cover any aspect of research into the broad topic of biological rhythms. The area covered can range from studies at the genetic or molecular level to those of behavioural or clinical topics. It can also include ultradian, circadian, infradian or annual rhythms. In this way, the Editorial Board tries to stimulate interdisciplinary rhythm research. Such an aim reflects not only the similarity of the methods used in different fields of chronobiology, but also the fact that many influences that exert controlling or masking effects are common. Amongst the controlling factors, attention is paid to the effects of climate change on living organisms. So, papers dealing with biometeorological aspects can also be submitted. The Journal publishes original scientific research papers, review papers, short notes on research in progress, book reviews and summaries of activities, symposia and congresses of national and international organizations dealing with rhythmic phenomena.