Determination of taurine in soft drinks by an ultrahigh-performance liquid chromatography-mass spectrometry method

European Pharmaceutical Journal Pub Date : 2023-08-31 DOI:10.2478/afpuc-2023-0010

P. Chalova, D. Salaskova, F. Csicsay, J. Galba, A. Kovac, J. Piestansky

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Abstract

Abstract Taurine (2-aminoethanesulfonic acid) is a free sulfur-containing β-amino acid widely distributed in many mammalians. Owing to the energizing effects, it is mostly used in soft drinks and supplements for athletes. Regular intake of soft drinks may lead to an overdose of caffeine, taurine, and guarana and loss of bone mass, overweight, hypertension, and in older age, osteoporosis and cardiovascular diseases. Therefore, it is essential to control the maximum amount of taurine consumed by humans in the food and beverages. Here, a fast, simple, accurate, and robust method based on ultrahigh-performance liquid chromatography hyphenated with mass spectrometry (UHPLC-MS) was successfully applied for the determination of taurine in selected soft drinks sold in Slovakia. The method was characterized by coefficient of determination higher than 0.99, and the predicted value of the limit of detection was 4.29 μmol/L. The analyzed levels of taurine in selected commercial drinks ranged from 2.8 to 3.78 mg/mL. The concentration in one brand of the investigated drinks was found to be extremely low (about 70%) compared to the declared content by the manufacturer.

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超高效液相色谱-质谱法测定软饮料中的牛磺酸

牛磺酸(2-氨基乙磺酸)是一种广泛存在于哺乳动物体内的游离含硫β-氨基酸。由于它的提神作用，它主要用于运动员的软饮料和补充剂中。经常饮用软饮料可能会导致咖啡因、牛磺酸和瓜拉那过量摄入，导致骨量减少、超重、高血压，在老年时还会导致骨质疏松和心血管疾病。因此，控制人体在食品和饮料中牛磺酸的最大摄取量至关重要。本文建立了一种快速、简单、准确、可靠的超高效液相色谱-质谱联用(UHPLC-MS)方法，成功地测定了斯洛伐克销售的软饮料中牛磺酸的含量。该方法的测定系数大于0.99，检出限预测值为4.29 μmol/L。选定的商业饮料中牛磺酸的分析水平在2.8至3.78毫克/毫升之间。被调查的一种饮料的浓度与制造商所声明的含量相比极低(约70%)。

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来源期刊

European Pharmaceutical Journal Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)

CiteScore

0.60

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0.00%

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期刊介绍： European Pharmaceutical Journal publishes only original articles not previously published and articles that are not being considered or have not been submitted for publication elsewhere. If parts of the results have been published as conference abstract or elsewhere, it should be stated in references. The ethical standards of the Helsinki-Tokio Declaration should be kept. This should be mentioned in the Methods of manuscript. Reviews are published only on request. Authors, whose submitted research work was performed with the support of a company, should indicate this in Conflict of Interest.