Potential Ekstract Of Red Betel Leaf (Piper crocatum Ruiz & Pav.) as a Natural Hand Sanitizer

Maya Monica Manurung, Tyas Hanurita Subekti, Nanda Dhea Ayu Sawitri, Iin Novita Nurhidayati Mahmuda
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Abstract

Background: Hands are the most common medium for spreading disease. Red betel leaf has antibacterial bioactive compounds such as flavonoids, alkaloids, polyphenols, tannins, and essential oils. The most common way to clean hands is to use hand sanitizer.Objective: To determine the antibacterial potential of red betel leaf extract (Piper crocatum Ruiz & Pav.) against the growth of Staphylococcus aureus, Streptococcus pyogenes, and Salmonella typhi as an active ingredient in hand sanitizers. Methods: Each bacterium, namely Staphylococus aureus, Streptococcus pyogenes and Salmonella typhi were divided into 6 groups, PEG 5% as a negative control, clindamycin for Staphylococus aureus and Streptococcus pyogenes and chloramphenicol for Salmonella typhi as a positive control, 70% alcohol as a comparison for hand sanitizer, extract 20%, 40%, and 80% as treatment groups. The obstacle zone is measured using a ruler.Results: Zones of inhibition against Staphylococus aureus, Streptococcus pyogenes, and Salmonella typhi were formed at concentrations of 20%, 40%, and 80%. Kruskal Wallis statistical analysis showed significant results with p=0.000.Conclusion: Red betel leaf extract has statistically significant antibacterial activity against Staphylococcus aureus, Streptococcus pyogenes and Salmonella typhi. The extract concentration of 40% is the optimal concentration as a raw material for hand sanitizer.
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红槟榔叶(Piper crocatum Ruiz &Pav.)作为天然洗手液
背景:手是传播疾病最常见的媒介。红槟榔叶含有抗菌生物活性化合物,如类黄酮、生物碱、多酚、单宁和精油。最常用的洗手方法是使用洗手液。目的:测定红槟榔叶提取物的抑菌潜力;(Pav.)作为洗手液中的一种有效成分,可以抑制金黄色葡萄球菌、化脓性链球菌和伤寒沙门氏菌的生长。方法:将每种细菌,即金黄色葡萄球菌、化脓性链球菌和伤寒沙门菌分为6组,5% PEG为阴性对照,5%林霉素为金黄色葡萄球菌、化脓性链球菌和氯霉素为伤寒沙门菌阳性对照,70%酒精为对照洗手液,提取液20%、40%和80%为治疗组。障碍物区域用尺子测量。结果:金黄色葡萄球菌、化脓性链球菌和伤寒沙门菌在浓度为20%、40%和80%时形成抑制区。Kruskal Wallis统计分析结果p=0.000。结论:槟榔叶提取物对金黄色葡萄球菌、化脓性链球菌和伤寒沙门氏菌具有显著的抑菌活性。提取液的浓度为40%是作为洗手液原料的最佳浓度。
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