Simultaneous sequencing of RNA by solid-phase chemical degradation using DE 81 anion-exchange paper

Burckhard Gross, André Rosenthal
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引用次数: 4

Abstract

A solid-phase chemical degradation method for simultaneous sequencing of RNA and RNA fragments has been developed using Whatman DE 81 anion-exchange paper as the support. The approach involves the following operations: (1) immobilization of the 3′-end labeled RNAs or RNA fragments on DE 81 paper; (2) washing; (3) modification reactions; (4) washing; (5) sorting of the paper segments; (6) aniline reaction; (7) lyophilization; (8) desorption of the RNA by TEAB; and (9) lyophilization.

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用de81阴离子交换纸固相化学降解法同时测序RNA
以Whatman DE 81阴离子交换纸为载体,建立了一种同时测序RNA和RNA片段的固相化学降解方法。该方法包括以下操作:(1)将3 '端标记的RNA或RNA片段固定在DE 81纸上;(2)洗涤;(3)改性反应;(4)清洗;(5)对纸段进行分类;(6)苯胺反应;(7)冻干法;(8) TEAB解吸RNA;(9)冻干。
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